产品描述The human asialoglycoprotein receptor (ASGPR) is an endocytic recycling receptor that belongs to the long-form subfamily of the C-type/Ca+2-dependent lectin family. ASGR1 is a liver-specific surface marker that is exclusively expressed in liver and mediates internalization and lysosomal degradation of blood asialoglycoproteins. 产品性质
Recombinant Human ASGR1 Protein is expressed from HEK293 Cells with His tag at the N-terminal. It contains Gln62-Leu291.
Molecular Weight
The protein has a predicted MW of 27.49 kDa. Due to glycosylation, the protein migrates to 38-55 kDa based on Tris-Bis PAGE result.
Purity
> 95% as determined by SDS-PAGE and HPLC
Endotoxin
< 1.0EU per 1μg of the protein by the LAL method.
Formulation
Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
Centrifuge tubes before opening. Reconstituting to a concentration more than100 μg/mL` is recommended (usually we use 1mg/mL solution for lyophilization). Dissolve the lyophilized protein in distilled water.
运输与保存方法The products are shipped with ice pack and can be stored at -20℃ for 1 year. 2-7 days, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 °C under sterile conditions after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please avoid freeze-thaw cycles. 注意事项
For your safety and health, please wear lab coats and disposable gloves for operation.