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- 翌圣生物（Yeasen）
- 93781ES60
- 上海
- 2026年02月09日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
现货
- 英文名：
Recombinant Human ASGR1 Protein, His Tag
- 保质期：
一年
- 供应商：
翌圣生物科技（上海）股份有限公司
- 保存条件：
-20度
- 规格：
100ug
产品性质
| Synonyms | ASGP-R 1; ASGPR 1; Hepatic lectin 1; HL-1; mHL-1; Asgr1; Asgr-1; |
| Uniprot No. | P07306-1 |
| Source | Recombinant Human ASGR1 Protein is expressed from HEK293 Cells with His tag at the N-terminal. It contains Gln62-Leu291. |
| Molecular Weight | The protein has a predicted MW of 27.49 kDa. Due to glycosylation, the protein migrates to 38-55 kDa based on Tris-Bis PAGE result. |
| Purity | > 95% as determined by SDS-PAGE and HPLC |
| Endotoxin | < 1.0 EU per 1μg of the protein by the LAL method. |
| Formulation | Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
| Reconstitution | Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/mL` is recommended (usually we use 1 mg/mL solution for lyophilization). Dissolve the lyophilized protein in distilled water. |
运输与保存方法The products are shipped with ice pack and can be stored at -20℃ for 1 year.
2-7 days, 2 to 8 °C under sterile conditions after reconstitution.
3 months, -20 °C under sterile conditions after reconstitution.
Recommend to aliquot the protein into smaller quantities for optimal storage. Please avoid freeze-thaw cycles.
注意事项
- For your safety and health, please wear lab coats and disposable gloves for operation.
- For research use only!
产品数据
HB221206
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文献和实验His 标签蛋白纯化效果不理想，宝宝心里苦呀。今天，小编来跟大家一起找找原因。 纯化所得组分中没有收集到重组的His标签蛋白。该问题主要可以分为以下两个方面： 1、His标签蛋白没有结合到填料上就流穿了 原因一 超声的功率不对。超声功率过高，容易导致蛋白炭化；功率过低，蛋白释放不出来。 建议 改变超声功率，同时可以在超声前加入溶菌酶。 原因二 结合缓冲液条件不合适。 建议 检查结合缓冲液中是否有影响结合的因素，如：金属离子螯合剂、强还原剂、过高的咪唑浓度。同时，优化缓冲液的pH、盐
植物源重组人胰岛素生长因子融合蛋白（OsrIGF-1），胰岛素在无血清细胞培养中的最佳替代品。 IGFs家族由两种低分子多肽(IGF-Ⅰ、IGF-Ⅱ)、两类特异性受体及六种结合蛋白组成。IGF-Ⅰ是一个有70个氨基酸的单链碱性蛋白，分子量7649Da，耐热。IGFs的生物学功能不只局限于有丝分裂刺激作用，它们也能诱导分化或促进分化功能的表达。全新推出的植物源重组人类胰岛素生长因子-1融合蛋白（OsrIGF-1）在IGF-1的N末端融合了人血清白蛋白（HSA），HSA作为蛋白质保护剂增加了IGF
Purification of 6xHis epitope tagged proteins by Ni-NTA-Agarose His标签蛋白纯化
crude sonicated sample Dispense into 1.5 ml eppendorf tubes,spin 14k rpm,4℃,30 min Combine soluble fractions into 1 tube Take 30μl soluble fraction as post-sonication soluble sample Test solubility of target protein by SDS-PAGE/Coomassie Blue Stain
