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- 文献和实验
- 技术资料
- 库存:
现货
- 英文名:
Recombinant SARS-COV-2 Spike RBD(Delta B.1.617.2) Protein, His Tag
- 保质期:
一年
- 供应商:
翌圣生物科技(上海)股份有限公司
- 保存条件:
-20度
- 规格:
25 μg(94041ES25)/100 μg(94041ES60)/500 μg(94041ES76)/1 mg(94041ES80)
| 规格: | 25 μg(94041ES25) | 产品价格: | ¥1295 |
|---|---|---|---|
| 规格: | 100 μg(94041ES60) | 产品价格: | ¥4585 |
| 规格: | 500 μg(94041ES76) | 产品价格: | ¥16855 |
| 规格: | 1 mg(94041ES80) | 产品价格: | ¥29855 |
产品描述:
Coronaviruses are commonly comprised of four structural proteins: Spike protein (S), Envelope protein (E), Membrane protein (M) and Nucleocapsid protein (N) . The SARS-CoV-2 S protein is a glycoprotein that mediates membrane fusion and viral entry. The S protein is homotrimeric, with each ~180-kDa monomer consisting of two subunits, S1 and S2 . The RBD of SARS-CoV-2 binds a metallopeptidase, angiotensin-converting enzyme 2 (ACE-2). First detected in India in December 2020, the B.1.617.2, or Delta variant, is considered a Variant of Concern (VOC) as it contains several mutations in the RBD domain that potentially affect viral fitness and transmissibility: L452R and T478K.
产品属性:
| 产品别称 | S protein RBD; S glycoprotein RBD; Spike protein RBD |
| 种属 | SARS-COV-2 |
| 表达序列 | Arg319-Phe541(L452R, T478K) |
| Accession | P₀DTC₂ |
| 标签 | His tag at the C-terminal |
| 表达系统 | HEK293 Cells |
| 分子量 | Approximately 26.26 kDa. Due to glycosylation, the protein migrates to 35-40 kDa based on Tris-Bis PAGE result. |
| 纯度 | > 95% as determined by SDS-PAGE and HPLC. |
| 内毒素 | < 1.0 EU per 1μg of the protein by the LAL method. |
| 制剂 | Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). |
| 外观 | 冻干粉 |
| 生物活性 | ELISA Data:Immobilized SARS-CoV-2 Spike RBD (Delta B.1.617.2) , His Tag at 1μg/ml (100μl/well) on the plate. Dose response curve for Human ACE2, hFc Tag with the EC50 of 9.8ng/ml determined by ELISA. |
| 复溶方法 | Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/mL is recommended (usually we use 1 mg/mL solution for lyophilization). Dissolve the lyophilized protein in distilled water. |
| Uniprot | P₀DTC₂ |
| 表达区间 | Arg319-Phe541(L452R, T478K) |
产品特色:
|
别名 |
S protein RBD; S glycoprotein RBD; Spike protein RBD |
|
Accession |
QHD43416.1 |
|
表达区间及表达系统 |
Recombinant SARS-COV-2 Spike RBD (Delta B.1.617.2) Protein is expressed from Expi293 with His tag at the C-terminal. It contains Arg319-Phe541(L452R, T478K). |
|
分子量 |
Approximately 26.26 kDa. Due to glycosylation, the protein migrates to 35-40 kDa based on Tris-Bis PAGE result. |
|
纯度 |
> 95% as determined by SDS-PAGE and HPLC. |
|
活性 |
ELISA Data:Immobilized SARS-CoV-2 Spike RBD (Delta B.1.617.2) , His Tag at 1μg/ml (100μl/well) on the plate. Dose response curve for Human ACE2, hFc Tag with the EC50 of 9.8ng/ml determined by ELISA. |
|
内毒素 |
< 1.0 EU per 1μg of the protein by the LAL method. |
|
制剂 |
Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). |
|
复溶 |
Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/mL is recommended (usually we use 1 mg/mL solution for lyophilization). Dissolve the lyophilized protein in distilled water. |
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