Product Description CXCL12 is belonging to the CXC chemokine family. SDF1 alpha and SDF1 beta cDNAs encode precursor proteins of 89 and 93 amino acid residues, respectively. SDF-1 is highly conserved between species, mouse CXCL12α shares approximately 93% amino acid sequence identity with human CXCL12α. CXCL12 is strongly chemotactic for T-lymphocytes, monocytes, but not neutrophils. Contrast to SDF-1β, SDF-1α is shorter by four amino acids at the C-terminal tail. Product Properties
Sterile Filtered White lyophilized (freeze-dried) powder.
Purity
>97% by SDS-PAGE and HPLC analyses.
Biological Activity
The biological activity determined by a chemotaxis bioassay using human peripheral blood monocytes is in a concentration range of 50-100 ng/mL. Fully biologically active when compared to standard.
Endotoxin
< 1.0 EU per 1μg of the protein by the LAL method.
Formulation
Lyophilized from a 0.2 μm filtered concentrated solution in PBS, pH 7.4.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20℃. Further dilutions should be made in appropriate buffered solutions.
Shipping and Storage The products are shipped with ice pack and can be stored at -20℃ for 1 year. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 °C under sterile conditions after reconstitution. Recommend to aliquot the protein into smaller quantities when first usedand avoid repeated freeze-thaw cycles. Cautions
Avoid repeated freeze-thaw cycles.
For your safety and health, please wear lab coats and disposable gloves for operation.