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- progen
- 德国
- 64008
- 2025年07月08日
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- 文献和实验
- 技术资料
- 保存条件：
Lyophilized at 2-8°C; reconstituted at -20°C (avoid freeze/thaw cycles)
- 库存：
1000
- 供应商：
PROGEN Biotechnik GmbH
- 规格：
50 µg
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文献和实验Protocol for anti-HA antibody Western Blotting
seconds, and pour it off: this method was worked out in the Yamamoto lab eons ago). 7) Incubate with secondary antibody (anti-mouse) in 5% BSA/PBS/Tween as you would ordinarily. I usually go for 2 hours @ room temp. 8) 12-15 quick washes again(in 1XPBS
% nonfat dry milk works well for anti-PCREB, but in my hands milk is not as good as BSA from USB (it seems to mask the epitope). Remove block solution and add primary antibody. For antiphosphoCREB (IgG, 0.7 mg/ml), I use 1:5,000
Western Blot 1、Run protein gel.Set up blot:2、Prepare two tupperware containers, one with dH2Oand one with 1/2X Transfer Buffer (TB).3、Remove one glass plate from the gel by twisting a spacer between the plates. To remove the other plate, place
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