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M13 antibody [05] (PE)

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  • ¥5600
  • GeneTex已认证
  • 美国
  • GTX03753-08
  • 2025年07月09日
  • FACS
  • Mouse
  • Bacteriophage M13
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    • 详细信息
    • 文献和实验
    • 技术资料
    • 免疫原

      M13 Bacteriophage

    • 亚型

      IgG1

    • 形态

      Liquid

    • 保存条件

      Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC. DO NOT FREEZE. Protect from light.

    • 克隆性

      Monoclonal

    • 标记物

      PE

    • 适应物种

      Bacteriophage M13

    • 保质期

      12 months from the shipping date of the product. 

    • 抗原来源

      Bacteriophage M13

    • 目录编号

      GTX03753-08

    • 级别

      Primary Antibodies

    • 库存

      Available

    • 供应商

      GeneTex

    • 宿主

      Mouse

    • 应用范围

      FACS

    • 浓度

      0.1 mg/ml (Please refer to the vial label for the specific concentration.)

    • 靶点

      M13

    • 抗体英文名

      M13 antibody [05] (PE)

    • 抗体名

      M13 抗体 [05] (PE)

    • 规格

      100 test

    产品细节图片1

    FACS analysis of 4-1BB-transfected CHO-K1 cells incubated with M13 phage particles (for 20 minutes) using GTX03753-08 M13 antibody [05] (PE).
    Filied profile: Primary antibody
    Open profile: Isotype control
    The histogram were derived from gated events with the forward and side light-scatter characteristics of intact cells.

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    图标文献和实验
    相关实验
    • Western Blotting with Horseradish Pe

      % MeOH). 3.For proteins larger than 120kDa, transfer to PVDF membrane at 1Amp constant current for 90 minutes or equivalent (250mAmp for 6 hours or 500mAmp for 3 hours) in transfer buffer + SDS (25mM Tris, 190mM glycine, 20% MeOH, 0.05% SDS

    • T-cell dependent antigen specific in situ staining (Pe)

      if you have them). Let them dry approx 1 hour in a dry atmosphere at RT. Antibodies (anti-Pe) in the sections degrade more rapidly than tissue antigens. Proceed with staining within 24 hours or freeze the slides. Antigen-specific staining: (Pe staining of Pe

    • Antibody cleanup

      to 0.05, and zymolyase (100T) to 60 ug/ml. Incubate 1 hour at 37oC. Pellet spheroplasts as above. Resuspend in 10 mls Solution A and pellet again. Combine the suspension of whole cells with the spheroplasts and pellet as above. Resuspend in 10

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