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- 2025年07月14日
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文献和实验Assembly of Nucleosomal Templates by Salt Dialysis
Because it is convenient to assemble nucleosomal templates through salt dialysis, large amounts of chromatin complexes can be made easily and in a short amount of time. This unit includes instructions for the various salt dialysis schemes (step versus gradient
C2C12 mouse muscle cells (plated 24 hr previous in 6‐well plates, 500,000 cells/well) Hank's balanced salt solution (HBSS
enzymes, incubation conditions and cut sites. Protocol 1. Prepare the reaction for restriction digestion by adding the following reagents in the order listed to a microcentrifuge tube: sterile ddH2 O q.s (where "q.s." means quantity sufficient) 10
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