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文献和实验Green Fluorescent Protein (GFP) as an Indicator of Transfection in Chicken Embryos
/negative genes can be introduced to study the effect of signal interference on cells. This basic protocol is concerned with the application of GFP as an indicator of transfection in chicken embryos. Lipofectin® Reagent (Life Technologies, catalog
The Immunology of DNA Vaccines
identification of tumor antigens (5 ), there has also been increasing interest in the development of DNA-based immunization for cancer. Preclinical studies demonstrate that DNA-based immunizations targeting model tumor antigens such as chicken ovalbumin (6 ), β
Preparation of Embryonic Extract
. 9. 25-50 ml Hank's balanced-salt solution, on ice. 10. 12-day chicken embryos, 12. 11. Polytron with PTA 10TS generator or a Dounce homogenizer with a Teflon pestle of coarse fitting. 12. 50 ml conical disposable centrifuge tubes. 13
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