AnaSpec AS-72263SensoLyte®SARS

AnaSpec AS-72263 SensoLyte®SARS-CoV-2类木瓜蛋白酶（PLpro）活性测定试剂盒*荧光法*，1 kit，SensoLyte® SARS-CoV-2 Papain-like Protease (PLpro) Activity Assay Kit *Fluorimetric*
AnaSpec ( 美国 )
AnaSpec公司成立于1993年，是一家为全球生命科学研究，提供综合蛋白质组学和基因组学解决方案的领先供应商。
公司产品主要分三大部分：提供专业多肽，检测试剂，抗体检测试剂盒；适合基础研究的高通量筛选和新药物研发相关试剂；多肽合成，抗体生产，寡核苷酸的合成等定制服务。
我们北京云肽生物科技有限公司作为AnaSpec公司的经销商为客户提供，正规进口，保证原装正品，货期稳定，折扣好的服务标准。
AnaSpec AS-72263 SensoLyte® SARS-CoV-2 Papain-like Protease (PLpro) Activity Assay Kit *Fluorimetric*，SensoLyte®SARS-CoV-2类木瓜蛋白酶（PLpro）活性测定试剂盒*荧光法*，1 kit，产品简介：
产品品牌：AnaSpec
产品名称：SensoLyte®SARS-CoV-2类木瓜蛋白酶（PLpro）活性测定试剂盒*荧光法*
英文名称：SensoLyte® SARS-CoV-2 Papain-like Protease (PLpro) Activity Assay Kit *Fluorimetric*
产品货号：AS-72263
产品规格：1 kit

AnaSpec AS-72263 SensoLyte® SARS-CoV-2 Papain-like Protease (PLpro) Activity Assay Kit *Fluorimetric*，SensoLyte®SARS-CoV-2类木瓜蛋白酶（PLpro）活性测定试剂盒*荧光法*，1 kit，产品说明：
SensoLyte®520 SARS-CoV-2类木瓜蛋白酶/去泛素酶活性检测试剂盒采用荧光肽底物检测去泛素化活性。当活性木瓜蛋白酶样蛋白酶切割底物时，在激发/发射=490 nm/520 nm时，会导致亮绿色荧光增加。该检测方法可检测样品中低至7.8 ng/mL的活性木瓜蛋白酶。该试剂盒可用于检测纯化酶制剂、生物样品中的酶活性，也可用于化合物筛选。
SARS-Cov-2木瓜蛋白酶样蛋白酶（PLpro）编码为非结构蛋白3（nsp3），参与病毒多蛋白的切割。这种酶水解泛素链，泛素链在炎症过程中起重要作用，并从蛋白质中去除干扰素刺激的基因15（ISG15）修饰以逆转抗病毒反应。PLpro作为SARS-CoV-2复制和感染宿主过程中的一种必需酶，已成为冠状病毒抑制剂开发的一个有吸引力的药物靶点。
The SensoLyte® 520 SARS-CoV-2 Papain-like Protease/ Deubiquitinase Activity Assay Kit employs a fluorescent peptide substrate for the detection of deubiquitnation activity. When active Papain-like Protease cleaves the substrate, it results in an increase of bright green fluorescence at excitation/emission = 490 nm/520 nm. This assay can detect as low as 7.8 ng/mL active Papain-like Protease in the sample. This kit can be used to detect enzyme activity in purified enzyme preparations, biological samples and can also be applied for the compound screening.

SARS-Cov-2 Papain-like Protease (PLpro) is encoded as non-structural protein 3 (nsp3) and it is involved in the cleavage of the viral polyprotein. This enzyme hydrolyzes ubiquitin chains which are important in the inflammation process and also removes interferon-stimulated gene 15 (ISG15) modifications from proteins to reverse antiviral responses. As an essential enzyme in the process of SARS-CoV-2 replication and infection of the host, PLpro has been an attractive drug target for the coronavirus inhibitor development.
Kits components  产品组件：
Component A: Papain-like Protease Deubiquitination substrate Ex/Em=490 nm/520 nm upon cleavage; 400µM, 50µL Component B: Rh110 fluorescence reference standard, Ex/Em=490 nm/520 nm; 400µM, 15µL Component C: Recombinant SARS-CoV-2 Papain-like protease; 0.1mg/mL, 10µL Component D: 2X Assay Buffer, 25mL Component E: Inhibitor (GRL0617); 10µM, 10µL
组分A：木瓜蛋白酶类去泛素化底物Ex/Em=490nm/520nm；400µM，50µL组分B:Rh110荧光对照品，Ex/Em=490 nm/520 nm；400µM，15µL组分C：重组SARS-CoV-2木瓜蛋白酶；0.1mg/mL，10µL组分D:2X分析缓冲液，25mL组分E：抑制剂（GRL0617）；10µM，10µL

AnaSpec品牌部分产品货号：
AnaSpec   AS-72262  SensoLyte® SARS-CoV-2 3CL Protease Activity Assay Kit *Fluorimetric*，      1 kit
AnaSpec   AS-72263  SensoLyte® SARS-CoV-2 Papain-like Protease (PLpro) Activity Assay Kit  1 kit
AnaSpec   AS-55551  SensoLyte® Anti-Human β-Amyloid (1-40) Quantitative ELISA Kit *Colorimetric* 1 kit
AnaSpec   AS-55552  SensoLyte® Anti-Human β-Amyloid (1-42) Quantitative ELISA Kit *Colorimetric* 1 kit
AnaSpec   AS-72246  AnaTag™ Europium Protein Labeling Kit      1 kit
AnaSpec   AS-72247  AnaTag™ Protein Labeling Kit for TR-FRET - 1 kit     1 kit
AnaSpec   AS-71120  SensoLyte® AMC Caspase Profiling Kit Fluorimetric - 1 kit 1 kit
AnaSpec   AS-71121  SensoLyte® AMC Caspase Substrate Sampler Kit Fluorimetric - 1 kit   1 kit
AnaSpec   AS-71124  SensoLyte® Green Protease Assay Kit Fluorimetric - 1 kit   1 kit
AnaSpec   AS-71126  SensoLyte® 490 HCV Protease Assay Kit Fluorimetric 500 assays - 1 kit      1 kit
AnaSpec   AS-71127  SensoLyte® 490 HIV Protease Assay Kit Fluorimetric - 1 kit 1 kit
AnaSpec   AS-71128  SensoLyte® 490 MMP-1 Assay Kit Fluorimetric - 1 kit   1 kit
AnaSpec   AS-71129  SensoLyte® 490 MMP-2 Assay Kit Fluorimetric - 1 kit   1 kit
AnaSpec   AS-72040  SensoLyte® 520 Renin Assay Kit Fluorimetric - 1 kit 1 kit
AnaSpec   AS-72122  SensoLyte® Luminescent Alkaline Phosphatase Assay Kit Luminometric - 1 kit  1 kit
AnaSpec   AS-72129  SensoLyte® 520 Thrombin Activity Assay Kit Fluorimetric - 1 kit    1 kit
AnaSpec   AS-20276  Beta-Amyloid (1-42) 1 mg
AnaSpec   AS-20276-25   Beta-Amyloid (1-42) 25 mg
AnaSpec   AS-20276-5     Beta-Amyloid (1-42) 5 mg
AnaSpec   AS-20291  Threonine Phosphopeptide, PKC Substrate 4, phosphorylated - 1 mg  1 mg
AnaSpec   AS-20292  Insulin Receptor (1142-1153), pTyr1146 - 1 mg   1 mg
AnaSpec   AS-21158  HA peptide     5 mg
AnaSpec   AS-24467  Exendin (9-39) 0.5 mg
AnaSpec   AS-24468  Exendin (9-39) 1 mg
AnaSpec   AS-64129-05   Beta-Amyloid (1-42) • HFIP    0.5 mg
AnaSpec   AS-64129-1     Beta-Amyloid (1-42) • HFIP    1 mg
AnaSpec   AS-72215  AggreSure ß-Amyloid (1-40), human - 0.25 mg  0.25 mg NET peptide
AnaSpec   AS-72216  AggreSure ß-Amyloid (1-42), human - 0.25 mg  0.25 mg NET peptide
AnaSpec   AS-72049  AnaTag™ HiLyte™ Fluor 647 Protein Labeling Kit - 1 kit 1 kit
AnaSpec   AS-72050  AnaTag™ HiLyte™ Fluor 647 Microscale Protein Labeling Kit - 1 kit 1 kit
AnaSpec   AS-72111  AnaTag™ APC Labeling Kit - 1 kit 1 kit
AnaSpec   AS-72113  AnaTag™ R - PE Labeling Kit - 1 kit   1 kit
AnaSpec   AS-55158-100 Recombinant human MOG (1-125)   100 µg
AnaSpec   AS-55158-1000     Recombinant human MOG (1-125)   1000 µg
AnaSpec   AS-55158-500 Recombinant human MOG (1-125)   500 µg
AnaSpec   AS-55525-1     MMP-12 (Catalytic Domain), human recombinant   1 µg
AnaSpec   AS-55525-10   MMP-12 (Catalytic Domain), human recombinant   10 µg
AnaSpec   AS-55525-50   MMP-12 (Catalytic Domain), human recombinant   50 µg
AnaSpec   AS-72237  Protein A-HiLyte™ Fluor 647 Conjugate - 1 mg  1 mg
AnaSpec   AS-72239  Protein A-HiLyte™ Fluor 750 Conjugate - 1 mg  1 mg
AnaSpec   AS-72237  Protein A-HiLyte™ Fluor 647 Conjugate - 1 mg  1 mg
AnaSpec   AS-72239  Protein A-HiLyte™ Fluor 750 Conjugate - 1 mg  1 mg
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