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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
Store at -20°C. Supplied in 50nM Tris-Glycine(pH 7.4), 0.15M NaCl, 40%Glycerol, 0.01% sodium azide and 0.05% BSA. Stable for 12 months from date of receipt.
- 适应物种:
Human, Mouse, Rat
- 保质期:
12个月
- 库存:
现货
- 宿主:
Rabbit
- 应用范围:
WB,IHC,ICC/IF,FC,IP
- 靶点:
Actin
- 规格:
20μL/50μL/100μL
| 规格: | 20μL | 产品价格: | ¥600.0 |
|---|---|---|---|
| 规格: | 50μL | 产品价格: | ¥1000.0 |
| 规格: | 100μL | 产品价格: | ¥1800.0 |
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文献和实验David Amberg Dialyze purified GST fusion proteins and actin into PBS + 1mM MgCl2 . Mix 5ug actin into 50ul total volume binding buffer. Mix 5ug GST-fusion protein into total volume 50ul binding buffer.
Protein purification; actin Overview ACTIN The most abundant muscle and non-muscle cytoskeletal protein. MW 42 kDa, 374/375 amino acids; various isoforms; structure resolved at 2.8 Angstrom. [see also protocol ID 207,208,209]
常用的β-actin 引物序列 human actin f ctc cat cct ggc ctc gct gt human actin r gct gtc acc ttc acc gtt cc product size:268 rabbit actin r agt gcg acg tgg aca tcc g rabbit actin f tgg ctc taa cag tcc gcc tag product size:295 mouse actin r cgt
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