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NIR-βgal-2
MCE 国际站：NIR-βgal-2
产品活性：NIR-βgal-2 是一种 β-半乳糖苷酶激活的近红外荧光探针，具有优异的灵敏度。NIR-βgal-2 可用于可视化乳腺癌中的 β-半乳糖苷酶。
研究领域：Others
作用靶点：Fluorescent Dye
In Vitro: NIR-βgal-2 displays superior sensitivity (detection limit = 2.0 × 10-3 U/mL), great affinity (Km = 1.84 μM), and catalytic efficiency (kcat/Km = 0.24 μM-1 s-1) for β-galactosidase.
The survival rate of cells (SKOV-3, 4T1, A549, and L02 cells) in the presence of NIR-βgal-2 is higher than 80% after 24 h, suggesting that the probe had low cytotoxicity toward the cells.
Human ovarian carcinoma cells (SKOV-3 cells) and mouse breast cancer cells (4T1 cells) that endogenously overexpress β-galactosidase are chosen as cell models for this study. In comparison, human normal liver cells (L02 cells) and human lung cancer cells (A549 cells) are selected as negative groups. After incubating with NIR-βgal-2 (5 μM) for 30 min, can observe bright fluorescence signals in SKOV-3 and 4T1 cells, whereas faint NIR fluorescence is observed in A549 and L02 cells. NIR-βgal-2 could differentiate efficiently and rapidly detect β-galactosidase in different cells and can distinguish cancer cells that overexpress β-galactosidase.
In Vivo: NIR-βgal-2 (tumor injection; 100 μM/25 μL; 0, 10, 20, 30, and 60 min; Ex=640 nm, Em=695-770 nm) observes an intense signal in the tumor area in 4T1-bearing mice.
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文献和实验MicroPET, MicroSPECT, and NIR Fluorescence Imaging of Biomolecules In Vivo
and quantifying the receptor expression in vivo by means of microPET, microSPECT, and NIR fluorescence.
and abdominal autofluorescence detected by in vivo fluorescence imaging of living mice. Mol Imaging. 2008 Jan-Feb;7(1):21-7.) 上图为测定每种食物颗粒的荧光强度。纯化饲料（purified diet）在绿色区域荧光最强，其次是无苜蓿饲料（Alfalfa-free diet）和常规饲料（regular diet）。相比之下，远红光和 NIR 区荧光最强的是常规饲料，无苜蓿饲料荧光较弱，纯化饲料荧
纳米颗粒外泌体的制备及体内注射 ACS Nano. 2017 Nov 28;11(11):10883-10893. 图 9 用金纳米颗粒和 PKH26 对外泌体进行双标记 ACS Nano. 2017 Nov 28;11(11):10883-10893. 四、用近红外（NIR）荧光探针进行标记： 这是一种使用两亲性探针对 NIR 荧光标记的外泌体进行内源性检测的方法，无需对外泌体进行免疫标记或合成或色谱处理。由于 NIR 波长的荧光探针能穿透体内组织，从而提供了从细胞直接转换到体内
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