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2353409-48-8
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TCO-PEG2-Sulfo-NHS ester-EDC Urea
CAS No. : 2353409-48-8
MCE 国际站：TCO-PEG2-Sulfo-NHS ester-EDC Urea
产品活性：TCO-PEG2-Sulfo-NHS ester-EDC Urea 是一种含有叠氮基团的点化学试剂。TCO-PEG2-Sulfo-NHS ester-EDC Urea 是一种 PEG 连接剂，包含一个 TCO 基团和一个磺基-NHS 酯基团。硫基使该分子可溶于含水的缓冲液。该试剂可用于标记具有 TCO 基团的抗体、蛋白质和其他含胺大分子。试剂级，仅供研究用。TCO-PEG2-Sulfo-NHS ester-EDC Urea 是一种点击化学试剂。它含有 TCO 基团，可以和含有 Tetrazine 基团的分子发生逆电子需求 Diels-Alder?反应 (iEDDA)。
研究领域：Antibody-drug Conjugate/ADC Related
作用靶点：ADC Linker
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文献和实验Determination of Protein Contacts by Chemical Cross‐Linking With EDC and Mass Spectrometry
.1 The mechanism of condensation (cross‐linking) reaction between a protein side chain carboxyl (I) and amine (II) group. (A ) Reaction with EDC alone. (B ) NHS forms a more stable active ester (V) and increases the yield of the isopeptide (cross‐link) bond (IV
and resuspend the washed microspheres in 80 μL 100 mM Monobasic Sodium Phosphate, pH 6.2 by vortex and sonication for approximately 20 seconds.7.Add 10 μL of 50 mg/mL Sulfo-NHS (diluted in dH20) to the microspheres and mix gently by vortex.8.Add 10 μL of 50 mg
Monobasic Sodium Phosphate, pH 6.2 by vortex and sonication for approximately 20 seconds. 9.Add 10 μL of 50 mg/mL Sulfo-NHS (diluted in dH2 0) to the microspheres and mix gently by vortex. 10.Add 10 μL of 50 mg/mL EDC (diluted in dH
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