- ￥2980 - 9800
- 攸碧艾/YOBIBIO
- U33-256A-F
- YOBIBIO
- 2026年04月14日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
1000
- 英文名：
/
- CAS号：
/
- 保质期：
1年
- 供应商：
上海攸碧艾生物
- 保存条件：
-20°C
- 规格：
1ml/5ml
| 规格： | 1ml | 产品价格： | ￥2980.0 |
|---|---|---|---|
| 规格： | 5ml | 产品价格： | ￥9800.0 |
Anti-HA agrose beads/Anti-HA Affinity Gel
PRODUCT NAME：Anti-HA agrose beads/Anti-HA Affinity Gel
CATALOG NUMBER： U33-256A-F
LOT NUMBER：U33022
ISOTYPE：IgG1
Clone NO：1B20
QUANTITY：1ml
DESCRIPTION：Anti-HA Affinity Gel is a purified murine IgG1 Monoclonal antibody covalently attached to sepharose 4B
APPLICATION：rProtein Purification, Immunoprecipitation
REAGENT：Supplied as a 50% suspension in 50% glycerol with 10mM sodium phosphate, 150mM sodium chloride, pH 7.4, containing 0.02% (w/v) sodium azide.
ANTIBODY BOUND：NTL 8.5 grams antibody/Liter gel
RECOMMENDED VOLUME：IP: 5μl gel for 5μl crude protein solution
BINDING CAPACITY：Minimum 1.5mg of a HA fusion protein eluted per ml of packed resin
SETTING VOLUME：2ml resin slurry
STORAGE/STABILITY：
Anti-HA Affinity Gel should be stored in 50% glycerol at -20℃ for maximum stability. The unopened product is stable for one year when stored as indicated. After use, the resin should be cleaned and stored in 50% glycerol with 10mM sodium phosphate, 150mM sodium chloride, pH 7.4, containing 0.02% sodium azide to protect the product. Do not freeze in the absence of glycerol.
PRODUCT NAME：Anti-HA agrose beads/Anti-HA Affinity Gel
CATALOG NUMBER： U33-256A-F
LOT NUMBER：U33022
ISOTYPE：IgG1
Clone NO：1B20
QUANTITY：5ml
DESCRIPTION：Anti-HA Affinity Gel is a purified murine IgG1 Monoclonal antibody covalently attached to sepharose 4B
APPLICATION：rProtein Purification, Immunoprecipitation
REAGENT：Supplied as a 50% suspension in 50% glycerol with 10mM sodium phosphate, 150mM sodium chloride, pH 7.4, containing 0.02% (w/v) sodium azide.
ANTIBODY BOUND：NTL 8.5 grams antibody/Liter gel
RECOMMENDED VOLUME：IP: 5μl gel for 5μl crude protein solution
BINDING CAPACITY：Minimum 1.5mg of a HA fusion protein eluted per ml of packed resin
SETTING VOLUME：2ml resin slurry
STORAGE/STABILITY：
Anti-HA Affinity Gel should be stored in 50% glycerol at -20℃ for maximum stability. The unopened product is stable for one year when stored as indicated. After use, the resin should be cleaned and stored in 50% glycerol with 10mM sodium phosphate, 150mM sodium chloride, pH 7.4, containing 0.02% sodium azide to protect the product. Do not freeze in the absence of glycerol.
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文献和实验monoclonal anti-HA 16B12 (MMS101R, BAbCo, used at 1:200 to 1:1000 dilution) mouse monoclonal anti-HA 12CA5 (12CA5, Boehringer, used at 1:200 dilution) rabbit polyclonal anti-HA (RS1010C, BAbCo, used at 1:200 dilution) Cy3-conjugated affinity
Anti-HA Immunocytology The multiple copies of the HA epitope present in the HAT tag can be detected by the mouse monoclonal antibodies 12CA5 (Boehringer) and 16B12 (MMS101R; BAbCO, Richmond, California) (see Table 1). On Western blots
Protocol for anti-HA antibody Western Blotting
of interest. 2) Transfer proteins in acrylamide gel to nylon membrane. 3) Block overnight @4℃ in PBS (or TBS, it doesn't matter) with 5% non-fat dry milk. I dilute 2.5 mg in 50 ml. 4) Wash off excess milk sol'n with PBS x3 .(important if you are re-using your antibody
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