- ￥2780 - 9380
- 攸碧艾/YOBIBIO
- U33-135A-F
- YOBIBIO
- 2026年01月13日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
1000
- 英文名：
/
- CAS号：
/
- 保质期：
1年
- 供应商：
上海攸碧艾生物
- 保存条件：
-20°C
- 规格：
1ml/5ml
| 规格： | 1ml | 产品价格： | ￥2780.0 |
|---|---|---|---|
| 规格： | 5ml | 产品价格： | ￥9380.0 |
Anti-Flag agrose beads/Anti-Flag Affinity Gel
PRODUCT NAME：Anti-Flag agrose beads/Anti-Flag Affinity Gel
CATALOG NUMBER： U33-135A-F
LOT NUMBER：U33022
ISOTYPE：IgG2b
Clone NO：1B20
QUANTITY：1ml
DESCRIPTION：Anti-Flag Affinity Gel is a purified murine IgG2b Monoclonal antibody covalently attached to sepharose 4B
APPLICATION：rProtein Purification, Immunoprecipitation
REAGENT：Supplied as a 50% suspension in 50% glycerol with 10mM sodium phosphate, 150mM sodium chloride, pH 7.4, containing 0.02% (w/v) sodium azide.
ANTIBODY BOUND：NLT 7.5 grams antibody/ Liter gel
RECOMMENDED VOLUME：IP: 5μl gel for 500μl crude protein solution
BINDING CAPACITY：Minimum 1.1mg of a Flag fusion protein eluted per ml of packed resin
SETTING VOLUME：10 ml resin slurry
STORAGE/STABILITY：
Anti-Flag Affinity Gel should be stored in 50% glycerol at -20℃ for maximum stability. The unopened product is stable for one year when stored as indicated. After use, the resin should be cleaned and stored in 50% glycerol with 10mM sodium phosphate, 150mM sodium chloride, pH 7.4, containing 0.02% sodium azide to protect the product. Do not freeze in the absence of glycerol
PRODUCT NAME：Anti-Flag agrose beads/Anti-Flag Affinity Gel
CATALOG NUMBER： U33-135A-F
LOT NUMBER：U33022
ISOTYPE：IgG2b
Clone NO：1B20
QUANTITY：5ml
DESCRIPTION：Anti-Flag Affinity Gel is a purified murine IgG2b Monoclonal antibody covalently attached to sepharose 4B
APPLICATION：rProtein Purification, Immunoprecipitation
REAGENT：Supplied as a 50% suspension in 50% glycerol with 10mM sodium phosphate, 150mM sodium chloride, pH 7.4, containing 0.02% (w/v) sodium azide.
ANTIBODY BOUND：NLT 7.5 grams antibody/ Liter gel
RECOMMENDED VOLUME：IP: 5μl gel for 500μl crude protein solution
BINDING CAPACITY：Minimum 1.1mg of a Flag fusion protein eluted per ml of packed resin
SETTING VOLUME：10 ml resin slurry
STORAGE/STABILITY：
Anti-Flag Affinity Gel should be stored in 50% glycerol at -20℃ for maximum stability. The unopened product is stable for one year when stored as indicated. After use, the resin should be cleaned and stored in 50% glycerol with 10mM sodium phosphate, 150mM sodium chloride, pH 7.4, containing 0.02% sodium azide to protect the product. Do not freeze in the absence of glycerol
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文献和实验and then centrifuge at 14,000 RPM for 3 minutes. Save 20µl of the elutes as (Flag-Input). Next, take the remaining ~80µl of elute to clean tubes containing 40µl of EZVIEW™ Red anti-Flag M2 affinity gel beads (Sigma™) which have been pre-treated with a wash in 250µl
Anti-DYKDDDDK tag (L5) Affinity Gel
or 0.22µm filter to prepare for L5 purification. Part II. Resin Preparation 1. Thoroughly suspend the resin by gentle inversion until the bottle of Anti- DYKDDDDK-tag (L5) affinity gel is a uniform suspension of gel beads. Transfer
stable line is resistant to both G418 and puromycin, and expresses FLAG‐tagged, biotinylated TF (FLBIO TF) that can be immunoprecipitated by anti‐FLAG and streptavidin antibodies/beads. View Image
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