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- 详细信息
- 文献和实验
- 技术资料
- 库存:
1000
- 英文名:
/
- CAS号:
/
- 保质期:
/
- 供应商:
UBI
- 保存条件:
-20°C
- 规格:
100ul/400ul
| 规格: | 100ul | 产品价格: | ¥1456.0 |
|---|---|---|---|
| 规格: | 400ul | 产品价格: | ¥3733.0 |
Anti-Flag Monoclonal Antibody
PRODUCT NAME:Anti-Flag Monoclonal Antibody
CATALOG NUMBER:U33-131A
LOT NUMBER:U33022
ISOTYPE:Mouse IgG2b
Clone NO:1B20
SOURCE:Mouse Ascites
QUANTITY:100ul
DESCRIPTION:Monoclonal antibodies are produced by immunizing mouse with synthetic peptide (DYKDDDDK), coupled to KLH.
APPLICATION:Western Blot, Immunoprecipitation
REAGENT:1×PBS with 50% glycerol
PROTEIN CONCENTRATION:0.5 mg/ml
RECOMMENDED ANTIBODY DILUTION:Western Blot: 0.5μg/ml
STORAGE/STABILITY: Antibody is recommended being stored at -20℃. Avoid repeated freeze-thaw cycle.
Background:
Epitope tags consisting of short sequences recognized by well-characterized monoclonal antibody have been widely used in study of protein expression in various systems. Our anti-flag monoclonal antibody provides a simple solution to detect the expression of protein with a Flag tag.
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文献和实验指仅对一种抗原决定基的纯粹的抗体。这类抗体不可能通过将抗原 胞的 Hybridoma,在试管内制成了纯粹的单克隆抗体。用某种抗原使动物产生免疫感应,对应于抗原之不同部分可制成各种不同的抗体复合体。然而,因为一个产生抗体的细胞,只能产生一种抗体,所以骨髓肿瘤细胞与产生抗体细胞间的细胞杂种,可形成边产生抗体边增殖的 hybridoma,将每个杂种细胞进行克隆(无性繁殖系)培养,则一个克隆的细胞群只由单种类产生同一抗体的细胞所组成,这样得到的就是单克隆抗体。此抗体在测定
Monoclonal Antibody Production
Order 6 six week old Balb/C mice and let the ARC know they are coming.Have your antigen ready for when they arrive.Once they get there earmark the mice and perform a pre-bleed on them to be used as an ELISA control for monitoring the titer
a simple ELISA method to detect human anti-antibody response.
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