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- 详细信息
- 文献和实验
- 技术资料
- 库存:
1000
- 英文名:
/
- CAS号:
/
- 保质期:
/
- 供应商:
UBI
- 保存条件:
-20°C
- 规格:
100ul
PRODUCT NAME:Anti-GAPDH-Peroxidase (HRP) Conjugated
CATALOG NUMBER:U33-841A-S
LOT NUMBER:U33022
ISOTYPE:Mouse IgG1
Clone NO:1B20
SOURCE:Mouse Ascites
PURIFICATION:Protein A purified
QUANTITY:100 μl
DESCRIPTION:Monoclonal antibody is produced by immunizing mouse with synthetic peptide [CRLEKPAKYDDIKK], coupled to KLH.
REACTIVITY:human, mouse, African green monkey
APPLICATIONS:Western Blot
BUFFER:10mM sodium phosphate, 150mM sodium chloride, pH 7.4 with 50% glycerol
PROTEIN CONCENTRATION:approximately 1 mg/ml
RECOMMMENED ANTIBODY DILUTION:Western blot: 1:4000
STORAGE/STABILITY:Antibody-HRP is recommended being stored at -20℃.
Background:
The enzyme glyceraldehydes-3-phosphate dehydrogenase is a tetramer of identical chains that catalyzes the reversible oxidative phosphorylation of glyceraldehydes-phosphate in the presence of inorganic phosphate and nicotinamide adenine dinucleotide. This is an important energy-yielding step in carbohydrate metabolism. GAPDH is found in almost all species with low rate of evolutionary changes.
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文献和实验【信息】Rabbit anti-GAPDH(SF-PA005)(兔抗 GAPDH 多克隆 IgG 抗体)招代理
Rabbit anti-GAPDH(SF-PA005)(兔抗 GAPDH 多克隆 IgG 抗体) 背景 甘油醛 -3- 磷 酸脱氢酶( Glyceraldehyde 3-phosphate dehydrogenase,GAPDH)是糖酵解(glycolysis)过程中的关键酶,分子量为37kDa 左右。它催化甘油醛-3-磷酸的氧化磷酸化反应。GAPDH 作为看家基因 在同种细胞 或者组织中一般是恒定、高水平表达的,常用作 Western Blot 实验的内参照. 来源 兔抗
:使用 Anti-phospho-Akt (Ser473) Rabbit mAb 对石蜡包埋的人乳腺癌组织进行免疫组织化学分析。(图 A)使用免疫组化试剂盒M&R HRP/DAB Detection IHC Kit,抗体 1:100 稀释;(图 B) 采用普通免疫组化试剂盒,抗体 1:25 稀释。 图 6 免疫组化实验检测 Erk1/2 表达 注:使用 Anti-Erk1/2 Mouse mAb与p44/42 MAPK (Erk1/2)Rabbit mAb 对正常小鼠心脏组织进行免疫
subunits. The molecular weight for GAPDH is 146 kDa. Since it is expressed in all cells it is for example becoming the marker of choice for a loading control in Western Blotting. The anti-GAPDH clone 6C5, which is developed at HyTest Ltd, gives very strong
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