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- 详细信息
- 文献和实验
- 技术资料
- 抗体名:
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- 抗体英文名:
,AKT1; PKB; RAC; RAC-alpha serine/threonine-protein kinase; Protein kinase B; PKB; Protein kinase B alpha; PKB alpha; Proto-oncogene c-Akt; RAC-PK-alpha
- 靶点:
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- 浓度:
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- 应用范围:
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- 宿主:
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- 适应物种:
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- 保质期:
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- 抗原来源:
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- 目录编号:
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- 级别:
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- 库存:
99
- 供应商:
爱必信(上海)生物科技有限公司
- 标记物:
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- 克隆性:
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- 保存条件:
Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
- 形态:
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- 亚型:
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- 免疫原:
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- 规格:
100ul
| 概述 | |
| 别名 | AKT1; PKB; RAC; RAC-alpha serine/threonine-protein kinase; Protein kinase B; PKB; Protein kinase B alpha; PKB alpha; Proto-oncogene c-Akt; RAC-PK-alpha |
| 宿主 | Mouse |
| 反应种属 | Human,Rat,Mouse |
| 应用 | IHC: 1/50-1/100 |
| 免疫原 | Synthetic peptide conjugated to KLH. |
| 性能 | |
| 形式 | Monoclonal Antibody |
| 纯化方法 | Affinity Purified |
| 克隆号 | 2A1 |
| 同种型 | IgG1 |
| 储存/保存方法 | Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. |
| 存储溶液 | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide, pH 7.3. |
| 修饰 | Unmodified |
| 靶标 | |
| 背景说明 | Akt, also referred to as PKB or Rac, plays a critical role in controlling survival and apoptosis. This protein kinase is activated by insulin and various growth and survival factors to function in a wortmannin-sensitive pathway involving PI3 kinase. Akt is activated by phospholipid binding and activation loop phosphorylation at Thr308 by PDK1 and by phosphorylation within the carboxy terminus at Ser473. |
| UniProt | P31749 |
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文献和实验米宝 这俩天做实验,极其郁闷。我做的总AKt和pAkt,大鼠脑组织,第一次跑总AKT的时候,我觉得可能是因为蛋白是提取的浓度比较好吧,出来了一条浓浓的条带,三组总AKt都出了,接下来做pAkt,就出了一个带,效果挺明显的。后来再做pAkt的时候,跑了4张膜,显影的时候,太干净了,上面什么都没有,甚至连个杂带,斑点 都没!实验是在实验员的领导下完成的,技术上基本没什么纰漏,查阅本版面有关这问题方面的介绍,有的解释说pakt降解了!一抗是用的Phospho-Akt
(adjust pH to 7.8 with Binding buffer; red color) to the Protein A column.Mouse antibodies of the IgG1 subclass do not have a high affinity for protein A. Purification on protein A beads using standard conditions will yield approximately 1/10
Purification of mAb (IgG) by Chang-Duk Jun, 03/14/2000 Purpose Materials Antibody 7E3 , 2L sup grown in flasks, frozen and thawed overnight. BioRad Affi-Gel Protein A MAPS II Buffers
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