- $265
- Ybscience
- Yb--3227
- 美国/德国/中国
- 2025年07月15日
- WB
- Mouse
- 详见说明书
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- 详细信息
- 文献和实验
- 技术资料
- 抗体名：
Akt1 Mouse mAb
- 抗体英文名：
Akt1 Mouse mAb
- 靶点：
详见说明书
- 浓度：
1.0mg/ml
- 应用范围：
WB
- 宿主：
Mouse
- 供应商：
上海钰博生物科技有限公司
- 库存：
大量
- 目录编号：
3227
- 抗原来源：
Mouse
- 保质期：
1年
- 适应物种：
详见说明书
- 标记物：
详见说明书
- 克隆性：
多克隆
- 保存条件：
Store at -20°C
- 形态：
液体或冻干粉
- 亚型：
详见说明书
- 免疫原：
详见说明书
- 规格：
50μg/50μl, 100μg/100μl
-
- Catalog No. : Yb--3227
- Size : 50μg/50μl, 100μg/100μl
-
- Mol Weight : 60KDa
- UniProt : P31749
-
- Isotype : Mouse IgG2a
- Clonality : Monoclonal
-
- Price : $265/100μg
- Availability : 3-4 days
Category
- Primary Antibodies
- Secondary antibody
- Loading control / tag antibody
Details
Alternative Names:
AKT; AKT1 kinase; C-AKT; EC 2.7.11.1; PKB; PKB-alpha; Protein kinase B; RAC; RAC-PK-alpha; RAC-alpha serine/threonine kinase, kinase Akt1;
Application:
WB
Reactivity:
Mouse,Transfected
Dilution:
WB 1:2000
 |
Western blot detection of AKT1 in 3T3 cell lysates using AKT1 mouse mAb (dilution 1:2000),with super ECL.Predicted band size:60KDa.Observed band size:60KDa. |
Purification:
Affinity-chromatography
Specificity:
Akt1 Mouse mAb detects endogenous levels of total Akt1
Immunogen:
Purified recombinant human AKT1 protein fragments expressed in E.coli.
Description:
AKT1 is one of 3 closely related serine/threonine-protein kinases (AKT1, AKT2 and AKT3) called the AKT kinase, and which regulate many processes including metabolism, proliferation, cell survival, growth and angiogenesis. This is mediated through serine and/or threonine phosphorylation of a range of downstream substrates.
Storage Condition and Buffer:
Mouse IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.
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文献和实验(adjust pH to 7.8 with Binding buffer; red color) to the Protein A column.Mouse antibodies of the IgG1 subclass do not have a high affinity for protein A. Purification on protein A beads using standard conditions will yield approximately 1/10
Purification of mAb (IgG) by Chang-Duk Jun, 03/14/2000 Purpose Materials Antibody 7E3 , 2L sup grown in flasks, frozen and thawed overnight. BioRad Affi-Gel Protein A MAPS II Buffers
T-Cell Activation Using mAb to CD3
One of the most common ways to assess T cell activation is to measure T cell proliferation upon in vitro stimulation of T cells via antigen or agonistic antibodies to TCR. This protocol is written as a starting point for examining in vitro proliferation of mouse
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