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- 爱必信(absin)
- 上海
- abs50029
- 2026年03月10日
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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
荧光染料需避光保存，2～8℃储存，收到货起有效期为12个月。
- 保质期：
12个月
- 英文名：
Absin 5-Color IHC Kit (Anti-Rabbit Secondary Antibody)
- 库存：
现货
- 供应商：
爱必信(上海)生物科技有限公司
- CAS号：
-
- 规格：
100T/20T/50T
| 规格： | 100T | 产品价格： | ￥22410.0 |
|---|---|---|---|
| 规格： | 20T | 产品价格： | ￥5890.0 |
| 规格： | 50T | 产品价格： | ￥13650.0 |
| 概述 | |
| 别名 | 多色试剂盒 |
| 描述 | 组织微环境中有复杂的细胞组成，这些细胞的表型、状态、丰度、分布都有着重要的生物学意义和临床价值。借助抗体染色可以将其在组织原位上呈现出来。免疫组化染色是一种研究组织形态和原位蛋白表达的常见技术，常规IHC检测只能展示单一指标，难以呈现复杂的组织微环境中的细胞组成、状态和关系，而这些信息对疾病的诊断和治疗至关重要！ 酪氨信号放大技术原理：类似常规免疫组化的DAB显色方法，TSA技术同样采用HRP标记的二抗，HRP催化加入体系的荧光素底物，生产活化荧光底物，活化底物可与抗原上的酪氨酸共价结合，使样品上稳定的共价结合荧光素。之后用热修复法洗去非共价结合的抗体，在换下一种一抗来第二轮孵育，换另一种荧光素底物，如此往复就可实现多重标记。 |
| 检验原理 | 多重荧光免疫组化染色是基于抗原、抗体特异性结合的原理，选用辣根过氧化物酶（HRP）标记的二抗抗体，对试剂盒中的荧光染料进行活化，将信号共价结合到抗原上。借助于不同的荧光染料标记，通过多轮染色循环实现组织或细胞的原位多靶点染色。 |
| 产品组成 | TSA单色荧光染料520、TSA单色荧光染料570、TSA单色荧光染料620、TSA单色荧光染料700、信号放大反应液、抗兔HRP标记二抗、抗荧光淬灭封片剂、DAPI. |
| 应用 | 主要用于组织、石蜡切片、TMA芯片的免疫组化染色。 |
| 使用方法 | 1.荧光染料的稀释：若染料为干粉，使用100ulDMSO溶解，制成100X染料母液；若染料为液态表示出厂即为DMSO溶解的100X母液；使用信号放大反应液按1:100稀释制备染料工作液（现用现配）；DAPI使用无菌水按1:100稀释制备工作液。 2.二抗的使用：该试剂盒搭配为抗兔二抗，实验前请核实一抗种属是否匹配。鼠兔通用二抗请参考（#abs50013）。 |
| 性能 | |
| 储存/保存方法 | 荧光染料需避光保存，2～8℃储存，收到货起有效期为12个月。 |
| 注意事项 | 1. 本试剂盒只用于免疫组化，不做其他用途。 2. 本试剂盒仅限于专业人员使用。 3. 应采用适当的防护措施，以避免试剂同皮肤和眼睛接触。 4. 超过有效期的试剂活性可能降低，因此不得使用超过有效期的试剂。 5. 若将本试剂盒的染色组分与其他公司的产品混合使用，在染色过程中可能出现异常情况。 6. 脱蜡不彻底，容易影响染色效果。 7. 为了防止可能出现的假阴性、假阳性结果，实验过程中需有阳性对照及阴性对照同时进行。 8. 使用本试剂盒中所产生的各种废弃物都应按照《医疗废物管理条例》进行处理。 |
| 参考文献 | |
| 参考文献 | 1. Mingzhu Yang, et al., Chemical-induced chromatin remodeling reprograms mouse ESCs to totipotent-like stem cells. Cell Stem Cell. 2022 Mar 3 2. Xin Guan, et al., Nanoparticle-enhanced radiotherapy synergizes with PD-L1 blockade to limit post-surgical cancer recurrence and metastasis. Nature Communication. 2022 May 20 3. Qian-Yue Zhang, et al., Lymphocyte infiltration and thyrocyte destruction are driven by stromal and immune cell components in Hashimoto’s thyroiditis. Nature Communications. 2022 Feb 9 4. Dabbs David J. 诊断免疫组织化学(M). 北京：北京大学医学出版社，2008.9. 5. 美】Ed Harlow, David Lane. 抗体技术指南(M). 北京：科学出版社，2002:79-80,105. 6. Stack, E. C., et al., Multiplexed immunohistochemistry, imaging, and quantitation: a review, with an assessment of Tyramide signal amplification, multispectral imaging and multiplex analysis. Methods, 2014 70 (1): 46-58. 7. 钱帮国. 焦磊. 多标记免疫荧光染色及多普成像技术在组织学研究中的应用. 中国组织化学与细胞化学杂志, 2017 (4): 373-382. |
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