- ￥2000
- 爱必信(Absin)
- 31.2 - 2000pg/mL
- 上海
- abs520010
- 2025年07月16日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
现货
- 供应商：
爱必信(上海)生物科技有限公司
- 检测范围：
31.2 - 2000pg/mL
- 检测方法：
双抗夹心法
- 应用：
-
- 适应物种：
-
- 标记物：
-
- 样本：
细胞上清液，血清，血浆
- 规格：
96T
|
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产品描述： 产品名称：Mouse TNF alpha ELISA Kit 中文别名: APC1 protein, Cachectin, Cachetin, DIF, TNF, TNF, monocyte-derived, TNFA, TNF-A, TNFalpha, TNF-alpha, TNF-alphacachectin, TNFATNF, macrophage-derived, TNFG1F, TNFSF1A, TNFSF2, TNFSF2TNF superfamily, member 2, tumor necrosis factor (TNF superfamily, member 2), tumor necrosis factor alpha, Tumor necrosis factor ligand superfamily member 2, tumor necrosis factor, tumor necrosis factor-alpha,小鼠肿瘤坏死因子αELisa试剂盒 描述: 检测原理： 本试剂盒采用双抗体夹心酶联免疫吸附检测技术。特异性小鼠 TNF-α抗体预包被在高亲和力的酶标板上。酶标板孔中加入标准品、待测样本和生物素化的检测抗体，经过孵育，样本中存在的TNF-α与固相抗体和检测抗体结合，形成免疫复合物。洗涤去除未结合的物质后，加入辣根过氧化物酶标记的链霉亲和素(Streptavidin-HRP)。洗涤后，加入显色底物，避光显色。加入终止液终止反应，在450 nm波长(参考校正波长540nm或570nm)测定吸光度值。 检测类型：双抗夹心法 形式：预包被96孔板 检测样本类型：细胞上清液，血清，血浆 上样量：100ul 试剂盒组分： 预包被96孔板、标准品、抗小鼠TNF-α检测抗体、稀释用缓冲液、显色液（A、B）、洗涤液、终止液、SA-HRP、封板膜和说明书一份。 灵敏度：5.5 pg/mL 检测范围：31.2 - 2,000 pg/mL 回收率范围：88-107% 保存方法：2-8℃ 标准曲线图 背景： 肿瘤坏死因子alpha（TNF-α），又称为cachectin和TNFSF1A，是肿瘤坏死因子超家族的原型配体。它是一个多效因子，在炎症反应、免疫系统发育、细胞凋亡和类脂代谢中起到一个中心作用。TNF-α也参与到许多病理过程，包括哮喘、克罗恩病、类风湿关节炎、神经性疼痛、肥胖症、II型糖尿病、感染性休克、自身免疫和癌症。人TNF-α是一个26 kDa的II型跨膜蛋白，由一个35个氨基酸（aa）胞内域、一个21 aa跨膜段和一个177 aa的胞外域（ECD）组成。在ECD区，人TNF-α和猕猴有97%氨基酸序列同源性，与牛、犬、棉鼠、马、猫、小鼠、猪、大鼠有71-92%的氨基酸序列同源性。它可以由多种不同细胞如免疫细胞、上皮细胞、内皮细胞、肿瘤细胞表达产生。TNF-α能诱导肿瘤细胞和病毒感染细胞的裂解，并与可溶性TNF RI结合，产生下游细胞的跨膜转到信号。TACE/ADAM17可引起含有TNF-α细胞膜的脱落从而释放具有活性的TNF-α细胞因子，它是由TNF-α胞外可溶性结构构成的三聚体，分子量为55 kDa。 TNF-α有两个受体：TNF RI和TNF RII。TNF RI分子量为55-60 kDa，表达广泛；TNF RII分子量为78-80 kDa，仅限于造血细胞表达。两者都是以同源三聚体形式表达；TNF-α和TNF RI和TNF RII结合的亲和力相似，可促进NFκB的激活 。然而，仅有TNF RI具有细胞死亡结构域，能引发细胞凋亡。这两种可溶性受体都被释放到人的血清和尿液中，并能中和TNF-α的活性。 absin，您身边的科研百宝箱 |
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