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文献和实验[资源] 申请上传 Current Protocols In Protein Science (60M) 见公共邮箱
5.15 Gene Expression Using the Vaccinia Virus/ T7 RNA Polymerase Hybrid System Unit 5.16 Choice of Cellular Protein Expression System Unit 5.17 Use of the Gateway System for Protein Expression in Multiple Hosts Chapter 6 Purification of Recombinant
Primer Premier: Program for Design of Degenerate Primers from a Protein Sequence
the Primer Premier primer design program. The surface antigen (HBsAg) protein sequence of the adw strain of hepatitis B virus (HBV) was reverse-translated using the program. The sequence was scanned, and primers were designed in regions of low degeneracy
our extensive experience with B-PER for isolating fusion proteins in which the fusion partner is either IPIII-His (an internal protein of bacteriophage T4 with a His tag at the C-terminus)or MBP (the E.coli maltose binding protein).The two cases illustrate
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