Anti-Alpha Smooth Muscle Actin

Isolation of Non-muscle Actin

by reaction with alkaline phosphatase-conjugated goat anti-mouse secondary antibody. Choose the actin pool broadly, however, consider also checking the column fractions by dot blot with mAB 3F2 to identify capping protein. Pool the actin fractions

平滑肌 smooth muscle，plain muscle

即无纹肌（ non-striated muscle）的通称。被视为较横纹肌原始的一种肌肉。平滑肌除作为无脊椎动物的躯体肌而有广泛分布外，在脊椎动物除心肌之外而大部分内脏肌也是由平滑肌组成的。虽有如斧足类的闭壳肌和足系牵引肌等是由平行走向长纤维状细胞（平滑肌纤维）所构成，但多数的平滑肌则是由长纺锤形（脊椎动物的内脏肌长不到 1毫米）的单核细胞构成。它不构成独立的器官，而只是成为构成体壁和内脏壁的因素（肌层）。其细胞实质仅由相当于横纹肌的向异性物质组成，整体表现同样的双折射。在有的平滑肌中可

LABELING MUSCLE ACTIN WITH IATR

,small vial. Procedure (perform under reduced light,4℃ unless otherwise noted) 1.Resuspend 10 mg lyophilized actin in 2 ml buffer 1.Be careful not to make bubbles. 2.Add DTT (100 mM stock)to 5 mM. 3.Dialyze against 250 ml buffer 1 overnight