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文献和实验A Stress Protein-Facilitated Antigen Expression System for Plasmid DNA Vaccines
In DNA vaccination, an exciting new immunization technique with potential applications in clinical medicine, expression plasmid DNA containing antigen-encoding sequences cloned under heterologous promoter control are delivered by techniques
of a promoter-probe plasmid that utilizes a variant of green fluorescent protein (GFP) as its reporter. The technique has been used successfully to identify novel Salmonella typhimurium genes that are selectively expressed following exposure to acid
Protein Expression and Purification Protocol
in a shaker (250 RPM) at 37o C until the OD @ 600 nm is 0.5 to 1.5 OD. (Anywhere in this range is fine, however, extremely dense starter cultures>1.5 OD can cause problems in protein expression. Under these dense growth conditions the plasmid
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