• 我要登录|
  • 免费注册
    |
  • 我的丁香通
    • 企业机构:
    • 成为企业机构
    • 个人用户:
    • 个人中心
  • 移动端
    移动端
丁香通 logo丁香实验_LOGO
搜实验

    大家都在搜

      大家都在搜

        0 人通过求购买到了急需的产品
        免费发布求购
        发布求购
        点赞
        收藏
        wx-share
        分享

        A Promoter Probe Plasmid Based on Green Fluorescent Protein: A Strategy for Studying Meningococcal Gene Expression

        互联网

        658
        Many bacterial genes are regulated in an environment-responsive fashion, and from the perspective of a pathogen, the host represents just another environment. Many genes that contribute to virulence are differentially expressed in response to host environments that they encounter during colonization and invasion ( 1 ). Recognition of this has led to the development of selection or reporter systems that utilize the increased activity of promoters during growth in vivo to identify genes that are selectively expressed during infection, and, thus, may contribute to the infection process ( 25 ). One of these techniques, differential fluorescence induction ( 2 , 3 ), involves the use of a promoter-probe plasmid that utilizes a variant of green fluorescent protein (GFP) as its reporter. The technique has been used successfully to identify novel Salmonella typhimurium genes that are selectively expressed following exposure to acid environments ( 3 ) and during infection of macrophages ( 2 ). GFP reporter systems have also been used to evaluate in vivo gene expression in other organisms including Staphylococcus aureus ( 6 ), Listeria monocytogenes ( 7 ), and Mycobacterium marinum ( 8 ). This chapter describes the use of the GFP-promoter-probe plasmid, pJSK411, which is suitable for the evaluation of differential gene expression in Neisseria meningitidis (Fig.1 ).
        Fig. 1.  Map of pJSK411 demonstrating restriction sites within the multiple cloning site. The binding site for the 401 US primer overlies the XhoI site and the 41 1DS primer binding site lies within the coding region of GFPmut3.

        ad image
        提问
        扫一扫
        丁香实验小程序二维码
        实验小助手
        丁香实验公众号二维码
        扫码领资料
        反馈
        TOP
        打开小程序