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- 文献和实验
- 技术资料
- 保存条件:
在凉爽的温度下储存。储存温度不超过-20°C。保持容器密闭,置于干燥通风处。
- 英文名:
Manuronic Block OLS. DP30-DP50 M/G ratio>4
- 规格:
10 mg
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文献和实验DNA Probe Purification Protocol
. 6. Discard liquid and centrifuge the empty column for 1 min 10,000 x g to dry the column matrix. This is critical for good DNA yields. 7. Place column into a clean 1.5 mL microcentrifuge tube. Add 30-50 ul (depending on desired concentration
How to Make Simple Solutions and Dilutions
as percent concentrations as weight per volume for dry reagent OR volume per volume for solutions. When working with a dry reagent it is mixed as dry mass (g) per volume and can be simply calculated as the concentration (expressed as a proportion or ratio) x
of H2O:54 g of Tris base27.5 g of boric acid20 ml of 0.5 M EDTA (pH 8.0)The 0.5x working solution is 45 mM Tris-borate/1 mM EDTA.TBE is usually made and stored as a 5x or 10x stock solution. The pH of the concentrated stock buffer should be approx
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