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- 翌圣生物（Yeasen）
- 50102ES02
- 上海
- 2026年03月31日
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- 详细信息
- 询价记录
- 文献和实验
- 技术资料
- 库存：
大量
- 英文名：
Dihydroethidium(DHE)
- CAS号：
38483-26-0
- 保质期：
2年
- 供应商：
翌圣生物科技（上海）股份有限公司
- 保存条件：
-20度
- 规格：
5mg
产品信息
| 产品名称 |
产品编号 |
规格 |
价格 |
| Dihydroethidium (DHE) 二氢乙锭（超氧化物阴离子荧光探针） |
50102ES02 |
5 mg |
477 |
| Dihydroethidium (DHE) 二氢乙锭（超氧化物阴离子荧光探针） |
50102ES25 |
25 mg |
1547 |
产品描述
Hydroethidine也称为Dihydroethidium，是一种最常用的超氧化物阴离子荧光检测探针,能有效地检测活性氧类。这种染料可以自由地进入细胞，在细胞内超氧化物阴离子作用下脱氢形成溴化乙锭。溴化乙锭可以与RNA或DNA结合产生红色荧光。当细胞内的超氧化物阴离子水平较高时，产生的溴化乙锭较多，红色荧光就较强，反之则较弱。这样就可以用二氢乙锭进行超氧化物阴离子水平的检测。
该探针已经广泛地用于NK细胞、中性粒细胞、内皮细胞、细胞HL60和巨噬细胞的研究，并且作为一种重要的染料用于鉴定肿瘤中细胞的增殖和低氧性。
产品性质
| 产品名称（Product Name） |
Dihydroethidium (Hydroethidium) |
| CAS号（CAS NO.） |
38483-26-0 |
| 分子式（Molecular Formula） |
C21H21N3 |
| 分子量（Molecular Weight） |
315.41 |
| 激发和发射波长（Ex/Em） |
518 nm/610 nm |
| 纯度( Purity ) |
≥90% |
| 外观（Appearance） |
红色粉末 |
| 溶解性（Solubility） |
溶于DMSO |
| 结构式（Structure） |
|
运输和保存方法
冰袋运输。粉末直接保存于-20℃，有效期2年。产品易被氧化，建议分装后-20℃避光密封保存，避免反复冻融，避光，保持干燥。
注意事项
1）请勿吸入、吞咽或者直接接触皮肤和眼睛。
2）二氢乙锭容易氧化且见光易分解，一定要避光干燥保存。
3）在没有细胞和外界诱导剂存在的情况下，溶解在培养液或者缓冲溶液中（PBS/ HBSS/ HEPES）的染料会发生水解，被空气或通过光诱导的方式氧化，而使溶液的荧光强度增加。
4）为了您的安全和健康，请穿实验服并戴一次性手套操作。
5）本产品仅用于科研用途，禁止用于人身上。
使用方法
1. 工作液的配制
本品以粉末形式提供，使用前需要经过短暂离心，用高纯度的DMSO制备成5~10 mM储液。分装后避光冻存于-20℃。使用前用缓冲液（PBS/ HBSS/ HEPES）稀释母液至所需工作浓度，通常5~20 μM就可以满足实验需求，具体的浓度要根据实验需要进行进一步的调整。
2. 染色
将细胞和染色液按照1:1的比例进行混合（例如，100 μL的细胞悬液加入100 μL的染色工作液），室温或37℃孵育60 min。
3. 荧光显微镜观察
二氢乙锭本身为蓝色荧光，最大激发波长为370 nm，最大发射波长为420 nm，脱氢后与RNA或DNA结合产生红色荧光，最大激发波长为300 nm，最大发射波长为610 nm，实际观察时也可以使用518 nm作为激发波长。
客户使用本产品发表的科研文献（部分）
[1] Feng L, et al. Neutrophil-like Cell-Membrane-Coated Nanozyme Therapy for Ischemic Brain Damage and Long-Term Neurological Functional Recovery. ACS Nano. 2021 Feb 23;15(2):2263-2280. doi: 10.1021/acsnano.0c07973. Epub 2021 Jan 11. PMID: 33426885. IF: 15.881
[2] [1] Rlabc D, et al. Inhibition of ROS activity by controlled release of proanthocyanidins from mesoporous silica nanocomposites effectively ameliorates heterotopic tendon ossification[J]. Chemical Engineering Journal, 2021. IF: 13.273
[3] Feng J, et al. AKAP1 contributes to impaired mtDNA replication and mitochondrial dysfunction in podocytes of diabetic kidney disease. Int J Biol Sci. 2022 Jun 13;18(10):4026-4042. doi: 10.7150/ijbs.73493. PMID: 35844803. IF: 10.75
[4] Chen L, et al. Therapeutic effect of SIRT3 on glucocorticoid-induced osteonecrosis of the femoral head via intracellular oxidative suppression. Free Radic Biol Med. 2021 Nov 20; 176:228-240. PMID: 34260898. IF: 7.376
[5] Qiao S, et al. Bergenin impedes the generation of extracellular matrix in glomerular mesangial cells and ameliorates diabetic nephropathy in mice by inhibiting oxidative stress via the mTOR/β-TrcP/Nrf2 pathway[J]. Free Radical Biology and Medicine, 2019, 145: 118-135. IF: 7.376
[6] Che Y, et al. The Combination of Rhodosin and MMF Prolongs Cardiac Allograft Survival by Inhibiting DC Maturation by Promoting Mitochondrial Fusion. Oxid Med Cell Longev. 2022 Jul 9; 2022:7260305. PMID: 35855862. IF: 7.31
[7] Hu J, et al. Inhibition of CACNA1H attenuates doxorubicin-induced acute cardiotoxicity by affecting endoplasmic reticulum stress[J]. Biomedicine & Pharmacotherapy, 2019, 120: 109475. IF: 6.529
[8] Lin Z, et al. Eicosapentaenoic Acid-Induced Autophagy Attenuates Intervertebral Disc Degeneration by Suppressing Endoplasmic Reticulum Stress, Extracellular Matrix Degradation, and Apoptosis. Front Cell Dev Biol. 2021 Nov 4; 9:745621. doi: 10.3389/fcell.2021.745621. PMID: 34805156; PMCID: PMC8599281. IF: 6.684
[9] Chen L, et al. Apelin-13 induces mitophagy in bone marrow mesenchymal stem cells to suppress intracellular oxidative stress and ameliorate osteoporosis by activation of AMPK signaling pathway. Free Radic Biol Med. 2021 Feb 1; 163:356-368. doi: 10.1016/j.freeradbiomed.2020.12.235. Epub 2020 Dec 30. PMID: 33385540. IF: 6.17
[10] Li D, et al. Cereulide Exposure Caused Cytopathogenic Damages of Liver and Kidney in Mice. Int J Mol Sci. 2021 Aug 24;22(17):9148. doi: 10.3390/ijms22179148. PMID: 34502057; PMCID: PMC8431326. IF：5.924
[11] Pan B, et al. Azilsartan Suppresses Osteoclastogenesis and Ameliorates Ovariectomy-Induced Osteoporosis by Inhibiting Reactive Oxygen Species Production and Activating Nrf2 Signaling. Front Pharmacol. 2021 Nov 26;12:774709. doi: 10.3389/fphar.2021.774709. PMID: 34899338; PMCID: PMC8662525. IF：5.811
[12] Wang K, et al. Icariin Prevents Extracellular Matrix Accumulation and Ameliorates Experimental Diabetic Kidney Disease by Inhibiting Oxidative Stress via GPER Mediated p62-Dependent Keap1 Degradation and Nrf2 Activation. Front Cell Dev Biol. 2020 Jul 17; 8:559. doi: 10.3389/fcell.2020.00559. PMID: 32766240; PMCID: PMC7379398. IF：5.186
[13] Zhu L, et al. The critical role of RasGRP4 in the growth of diffuse large B cell lymphoma. Cell Commun Signal. 2019 Aug 13;17(1):92. doi: 10.1186/s12964-019-0415-6. PMID: 31409422; PMCID: PMC6693169. IF：5.128
HB220909
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- 作者
- 内容
- 询问日期
文献和实验[1] Feng L, et al. Neutrophil-like Cell-Membrane-Coated Nanozyme Therapy for Ischemic Brain Damage and Long-Term Neurological Functional Recovery. ACS Nano. 2021 Feb 23;15(2):2263-2280. doi: 10.1021/acsnano.0c07973. Epub 2021 Jan 11. PMID: 33426885. IF: 15.881
[2] [1] Rlabc D, et al. Inhibition of ROS activity by controlled release of proanthocyanidins from mesoporous silica nanocomposites effectively ameliorates heterotopic tendon ossification[J]. Chemical Engineering Journal, 2021. IF: 13.273
[3] Feng J, et al. AKAP1 contributes to impaired mtDNA replication and mitochondrial dysfunction in podocytes of diabetic kidney disease. Int J Biol Sci. 2022 Jun 13;18(10):4026-4042. doi: 10.7150/ijbs.73493. PMID: 35844803. IF: 10.75
[4] Chen L, et al. Therapeutic effect of SIRT3 on glucocorticoid-induced osteonecrosis of the femoral head via intracellular oxidative suppression. Free Radic Biol Med. 2021 Nov 20; 176:228-240. PMID: 34260898. IF: 7.376
[5] Qiao S, et al. Bergenin impedes the generation of extracellular matrix in glomerular mesangial cells and ameliorates diabetic nephropathy in mice by inhibiting oxidative stress via the mTOR/β-TrcP/Nrf2 pathway[J]. Free Radical Biology and Medicine, 2019, 145: 118-135. IF: 7.376
[6] Che Y, et al. The Combination of Rhodosin and MMF Prolongs Cardiac Allograft Survival by Inhibiting DC Maturation by Promoting Mitochondrial Fusion. Oxid Med Cell Longev. 2022 Jul 9; 2022:7260305. PMID: 35855862. IF: 7.31
[7] Hu J, et al. Inhibition of CACNA1H attenuates doxorubicin-induced acute cardiotoxicity by affecting endoplasmic reticulum stress[J]. Biomedicine & Pharmacotherapy, 2019, 120: 109475. IF: 6.529
[8] Lin Z, et al. Eicosapentaenoic Acid-Induced Autophagy Attenuates Intervertebral Disc Degeneration by Suppressing Endoplasmic Reticulum Stress, Extracellular Matrix Degradation, and Apoptosis. Front Cell Dev Biol. 2021 Nov 4; 9:745621. doi: 10.3389/fcell.2021.745621. PMID: 34805156; PMCID: PMC8599281. IF: 6.684
[9] Chen L, et al. Apelin-13 induces mitophagy in bone marrow mesenchymal stem cells to suppress intracellular oxidative stress and ameliorate osteoporosis by activation of AMPK signaling pathway. Free Radic Biol Med. 2021 Feb 1; 163:356-368. doi: 10.1016/j.freeradbiomed.2020.12.235. Epub 2020 Dec 30. PMID: 33385540. IF: 6.17
[10] Li D, et al. Cereulide Exposure Caused Cytopathogenic Damages of Liver and Kidney in Mice. Int J Mol Sci. 2021 Aug 24;22(17):9148. doi: 10.3390/ijms22179148. PMID: 34502057; PMCID: PMC8431326. IF：5.924
抗逆从而抑制了活性氧等胁迫。 ikewei 可以试试用DHE染料去测一下超氧阴离子的变化，有文献报道细胞外的过氧化氢做用细胞后，细胞内的超氧阴离子升高，而过氧化氢不怎么变。帮你找了下文献 Superoxide is the major reactive oxygen species regulating autophagy. ----------Cell Death Differ. 2009 Jul;16(7):1040-52. Epub
