BMP-4

Product Sheet

BMP-4, human recombinant
Catalog # CB031-0461、CB031-0462、CB031-0463
Description Bone morphogenetic proteins (BMPs) constitute a subfamily within the TGF-_ family of structurally related signaling proteins. BMP-4 is involved in the development and maintenance of bone and cartilage. Recombinant human BMP-4, expressed in mammalian cells, is a 32-38 kDa homodimeric glycoprotein.ExCell's BMP-4 is made in human 293 cells, which renders human-specific post-translational modifications. We purified this product through a series of chromatography including HPLC.
Source Human 293 cells
Formulation Lyophilized in sterile filtered solution of acetonitrile/TFA/NaCl solution.
Reconstitution Before reconstitution, we recommend a brief spin to drive down any material dislodged from the bottom of the tube. The lyophilized protein should be reconstituted in 10 mM Acetic Acid or 5 mM HCl. Further dilutions should be made in buffer or medium containing carrier proteins,such as albumin or serum.
Stability The lyophilized protein is stable for at least 1 year if stored at -80 degree C.Reconstituted protein is stable for at least 1 month at 4 degree C, but should be stored in aliquots at -80 degree C for longer term. Avoid repeated freeze/thaw.
Purity Greater than 95% by SDS-PAGE and HPLC analysis
Biological Activity The activity was determined by using a BMP response element (BRE) reporter gene assay. The EC50 ranges from 1 - 5 ng/ml.
Country of Origin China
For Research Use Only. Not for Use in Humans.