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1000ug
Product Sheet
Insulin, human recombinantCatalog # CB031-0071
Description The human insulin is the most important factor in controlling blood glucose level. It is also used to promote proliferation of cultured cells, including stem cells.This product is produced in Escherichia coli as a double chain, nonglycosylated,polypeptide containing 60 amino acids with a molecular mass of 5.8 kDa.
This product is suitable for tissue culture uses.
Source Escherichia coli. Endotoxin level of this product is lower than 0.1 EU/ug.
Formulation Lyophilized from a sterile filtered solution of salt and glycerol, carrier-free.
Reconstitution Before reconstitution, we recommend a brief spin to drive down any material dislodged from the bottom of the tube. It is recommended to reconstitute the lyophilized product in 0.1N acetic acid.
Stability The lyophilized protein is stable for at least 1 year if stored at -80 degree C.Reconstituted protein is stable for at least 2 months at 4 degree C. Avoid repeated freeze/thaw.
Purity Greater than 95% by HPLC analysis
Biological Activity The activity was determined by culturing human cells.
For Research Use Only. Not for Use in Humans.
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文献和实验Secretion of Recombinant Human Insulin-Like Growth Factor I (IGF-I)
The development of efficient recombinant protein production processes can be a critical factor in whether or not a pharmaceutical therapeutic protein can enter human clinical trials and ultimately the marketplace. This is especially true
Generation of Functional Insulin-Producing Cells from Human Embryonic Stem Cells In Vitro
system, which could induce human ES cells to differentiate into functional insulin-producing cells. Activin A was first used in the initial stage to induce definitive endoderm lineage differentiation from human ES cells. And all-trans Retinoic Acid (RA) was then utilized
Analysis of Insulin and Insulin-Like Growth Factor-I Receptors in Neural Tissues
A variety of biochemical and physiological studies in recent years have made it clear that the brain possesses specific receptors and puta- tive signal transduction pathways for insulin and the insulin-like growth factors (IGF-I and IGF
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