- ￥3392
- Merck-Calbiochem
- 116815-10UMOL
- 2026年05月29日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
大量
- 英文名：
cAMPS Sp-Isomer TEA Salt
- 供应商：
Merck
- 规格：
10UMO
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文献和实验posttranslational modifications of histones, and the cumbersome use of reverse-phase high-performance liquid chromatography. The three methodologies are shorter than the conventionally used protocols. The salt-urea method exploits the stability of the cell nucleus
EDTA). This is then placed into a large volume (e.g. 4 litres) of low-salt buffer (10mM TEA-HCl (comment 2) pH 7.4, 1mM EDTA) and left to dialyse overnight at +4ºC. The TEA �HCl buffer is used in the reconstitution to permit glutaraldehyde cross-linking
the crude cell lysate onto SP column, wash and then elute using a salt concentration gradient on the FPLC (programme 5). 5) Analyse the eluate with SDS-PAGE and pool the desired fractions. 6) Equilibrate the gel-filtration column with 20 mM
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