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- 详细信息
- 技术资料
- 免疫原:
A synthetic peptide from the internal region of EML4-ALK of human origin.
- 亚型:
IgG
- 形态:
液体
- 保存条件:
零下20℃
- 克隆性:
单克隆
- 标记物:
FITC
- 适应物种:
脊椎动物
- 保质期:
24个月
- 抗原来源:
蛋白
- 目录编号:
TD-26242
- 级别:
科研级
- 库存:
999
- 供应商:
武汉天德
- 宿主:
Mouse
- 应用范围:
ELISA, WB, IHC
- 浓度:
1mg/ml
- 靶点:
EML4-ALK
- 抗体英文名:
Anti EML4-ALK Mouse Monoclonal Antibody
- 抗体名:
EML4-ALK
- 规格:
100μl
EML4-ALK
| Catalog Number: TD-26242 |
| Gene Symbol: EML4-ALK |
| Description: Anti EML4 ALK Mouse Monoclonal Antibody |
| Background: EML4-ALK is a fusion-type protein kinase that can be found in many tumors, such as anaplastic large cell lymphoma, inflammatory myofibroblastic tumor, neuroblastoma and NSCLC, and so on. It is generated as a result of a small inversion within the short arm of human chromosome 2. EML4-ALK protein often promotes and maintains the malignant behavior of the cancer cells by activating the MAPK, PI3K/AKT and JAK/STAT3 pathways. |
| Immunogen: A synthetic peptide from the internal region of EML4-ALK of human origin. |
| Applications: ELISA, WB, IHC |
| Recommended Dilutions: ELISA: 1:1000-1:2000 、WB: 1:500-1:1000 、IHC: 1:50-1:100 |
| Concentration: 1 mg/ml |
| Host: Mouse |
| Format: Liquid |
| Clonality: Monoclonal |
| Isotype: |
| Purity: Purified from ascites |
| Storage buffer: |
| Preservative: no |
| Constituents: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol |
| Species Reactivity: Recognizes T41A mutant, but not wild-type EML4-ALK of vertebrates. |
| Storage Conditions: Store at -20°C. Avoid freeze / thaw cycles |
| Western blot: |
![]() |
Western blot analysis of recombinant EML4 ALK and wildtype proteins. Purified His-tagged EML4-ALK protein (lane 3), corresponding wild-type EML4 protein (lane1) and wild-type ALK protein (lane2) were blotted with Anti EML4 ALK monoclonal antibody (Cat. #TD-26242). |
| Immunofluorescence: |
![]() | Immunofluorescence of cells expressing EML4-ALK proteins with Anti EML4 ALK antibody. HEK293T cells were transfected with pCDNA3-GFP-EML4 (WT) plasmid (left column) or pCDNA3-GFP ALK(WT) plasmid (middle column) or pCDNA3-GFP-EML4-ALK plasmid (right column), then fixed and stained with Anti EML4 ALK monoclonal antibody (Cat. #TD-26242). |
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EML4-ALK
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