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- 详细信息
- 技术资料
- 供应商:
武汉天德
- 库存:
999
- 靶点:
ATM
- 级别:
科研级
- 目录编号:
TD-21276
- 克隆性:
多克隆
- 抗原来源:
蛋白
- 保质期:
24个月
- 抗体英文名:
Anti-ATM Rabbit polyclonal antibody
- 抗体名:
ATM Polyclonal Antibody
- 标记物:
详询
- 宿主:
兔
- 适应物种:
脊椎动物
- 免疫原:
Purified ATM protein, human origin.
- 亚型:
IgG
- 形态:
液体
- 应用范围:
ELISA, WB, IHC
- 保存条件:
-20℃
- 浓度:
0.2mg/ml
- 规格:
100μl
ATM Polyclonal Antibody
| Cat. #: TD-21276 |
| Gene Symbol: ATM |
| Description: Anti-ATM Rabbit Polyclonal Antibody |
| Background: Ataxia telangiectasia mutated (ATM) is a serine/threonine protein kinase that is recruited and activated by DNA double-strand breaks. It phosphorylates several key proteins that initiate activation of the DNA damage checkpoint, leading to cell cycle arrest, DNA repair or apoptosis. Activity of ATM protein is under tight control, and mutation of ATM can cause disease such as Ataxia telangiectasia (AT) and cancers. |
| Immunogen: Purified ATM protein, human origin. |
| Applications: ELISA, WB, IHC |
| Recommended Dilutions: ELISA: 1:1000-1:5000 WB: 1:100-1:1000 IHC: 1:50-1:200 |
| Concentration: 0.2 mg/ml |
| Host Species: Rabbit |
| Format: Liquid |
| Clonality: Polyclonal |
| Isotype: |
| Purity: Purified from serum |
| Preservative: No |
| Constituents: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol |
| Species Reactivity: Recognizes ATM of vertebrates. |
| Storage Conditions: Store at -20°C. Avoid repeated freezing and thawing |
Western blot:

Western blot analysis of recombinant ATM protein. Purified His-tagged ATM protein was blotted with Anti-ATM rabbit polyclonal antibody (Cat. #TD-21276).
Immunohistochemistry:

Immunohistochemical analysis of paraf\fin-embedded human thyroid cancer tissue with Anti-ATM polyclonal antibody (Cat. #TD-21276). Tissue samples were fixed with formaldehyde and blocked with 1% serum for 15 min at 37 °C. Antigen retrieval was by heat mediation in citrate buffer (pH6). Samples were then incubated with primary antibody (1:50 dilution) overnight at 4°C. A HRP-conjugated Goat anti-mouse IgG (1:50 dilution) was used as secondary antibody.
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