- ￥4800
- Tanda
- 美国
- TD-26305
- 2026年01月23日
- ELISA, WB, IHC
- Mouse
- 脊椎动物
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
A synthetic peptide from the internal region of CTNNB1 which includes the mutation of T41A, human origin.
- 亚型：
IgG
- 形态：
液体
- 保存条件：
零下20℃
- 克隆性：
单克隆
- 标记物：
FITC
- 适应物种：
脊椎动物
- 保质期：
24个月
- 抗原来源：
蛋白
- 目录编号：
TD-26305
- 级别：
科研级
- 库存：
999
- 供应商：
武汉天德
- 宿主：
Mouse
- 应用范围：
ELISA, WB, IHC
- 浓度：
1mg/ml
- 靶点：
T41A
- 抗体英文名：
Anti CTNNB1(T41A) Mouse Monoclonal Antibody
- 抗体名：
CTNNB1(T41A)
- 规格：
100μl
CTNNB1(T41A)
| Cat. #: TD-26305 |
| Gene Symbol: Beta-catenin, CTNNB, Catenin beta-1 |
| Description: Anti-CTNNB1(T41A) Mouse Monoclonal Antibody |
| Background: CTNNB1 protein is a dual function protein. It is a subunit of a complex of proteins that from adherent junctions, which are important for the establishment and maintenance of epithelial cell layers by regulating cell growth and adhesion between adjacent cells. CTNNB1 protein also pulls double duty as an intracellular signal transducer in the Wnt signaling pathway. Mutations of CTNNB1 have been implicated in the pathogenesis of several cancers. |
| Immunogen: A synthetic peptide from the internal region of CTNNB1 which includes the mutation of T41A, human origin. |
| Applications: ELISA, WB, IHC |
| Recommended Dilutions: ELISA: 1:1000-1:5000 WB: 1:500-1:2000 IHC: 1:50-1:100 |
| Concentration: 1 mg/ml |
| Host Species: Mouse |
| Format: Liquid |
| Clonality: Monoclonal |
| Isotype: IgG |
| Purity: Purified from ascites |
| Preservative: No |
| Constituents: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol |
| Species Reactivity: Recognizes T41A mutant, but not wild type CTNNB1 of vertebrates. |
| Storage Conditions: Store at -20°C. Avoid repeated freezing and thawing |
|
Western blot:
Western blot analysis of recombinant CTNNB1 (T41A) and wild type proteins. Purified His-tagged CTNNB1 (T41A) protein (lane2) and corresponding wild type protein (lane1) were blotted with Anti-CTNNB1(T41A) monoclonal antibody (Cat. #TD-26305).
Immunofluorescence of cells expressing CTNNB1 proteins with Anti-CTNNB1(T41A) antibody. HEK293T cells were transfected with pCDNA3-GFP-CTNNB1 (WT) plasmid (left column) or pCDNA3-GFP-CTNNB1 (T41A) plasmid (right column), then fixed and stained with Anti-CTNNB1(T41A) monoclonal antibody (Cat. #TD-26305). |
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文献和实验DNA extraction from Mutation Detection Enhancement (MDE) Gel Stained with Silver Nitrate
successfully to improve mutation detection in exon3 of the ¦Â-catenin (CTNNB1) gene in gastric adenocarcinoma by PCR-SSCP method. Following extraction of genomic DNA from paraffin embedded tissues (7), 5 to 10µl of the crude extract were amplified in 25 µl
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