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- 详细信息
- 文献和实验
- 技术资料
- 库存：
50
- 英文名：
Strep-Tag II mouse monoclonal antibody
- 供应商：
上海博尔森生物科技有限公司
- 保存条件：
-20℃
- 规格：
100μL
Strep-Tag II mouse monoclonal antibody
Streptavidin is a tetrameric protein purified from Streptomyces avidinii. It has wide used in numerous molecular
biological protocols dues to its strong affinity for biotin. NWSHPQFEK (Strep tag II) is a nine amino acid peptide
with high specificity and affinity towards streptavidin. NWSHPQFEK Tag Antibody is a useful tool in analysis
and affinity purification of Strep tag II fusion proteins. Product Name: STREP-tag mouse monoclonal antibody
Isotype: IgG1
Tested applications and dilutions: WB 1:2000-5000 IP 1:200
Produce anti- Strep tag II antibody affinity column purify Strep tag II recombinant proteins
Form: Liquid
Concentration: 1mg/ml in PBS, pH 7.4, containing 0.02% sodium azide and 50% glycerol
Storage: STREP-tag antibody is stable for 1 month at 2-8°C. For long term storage, Aliquot and store at -20°C or
below. Avoid repeated freezing and thawing cycles. Alternative Names: Strep Tag II antibody, NWSHPQFEK Tag Antibody
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文献和实验Monoclonal Antibody Production
and screening of the hybridomas: 1. Bleeding Mice (1) Place the mouse in a mouse restrainer. (2) Sterilize the tail with 70% ethanol. (3) With a razor blade,nip off the last 2 mm of the tip of the tail. (4) Using a milking motion,pull blood down and let
Purification and Analysis of Strep-tagged Antibody-Fragments
The Strep-tag is a nine-residue minimal peptide sequence with intrinsic affinity towards streptavidin. This peptide – or its improved version Strep-tag II – can be fused to recombinant antibody fragments and other proteins in various fashions
Monoclonal Antibody Production Protocol
complete with MCM instead of PCM (= IMDM - m ), and keep it in the incubator for fused cells. Isolation of spleen cells Sacrifice mouse by cervical dislocation. Immerse mouse in 70% Ethanol. Remove spleen ( on the left side ) and transfer into a small
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