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- 禾午生物
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- P41965
- 2026年09月06日
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- 文献和实验
- 技术资料
- 保存条件：
负20℃&负80℃
- 保质期：
3个月~12个月
- 英文名：
pU6-NONO(mouse)-sgRNA1-sgRNA2-sgRNA3-CBH-Cre-HA
- 库存：
99
- 供应商：
禾午生物
- 规格：
干粉&甘油菌
| 启动子 | U6 |
| 复制子 | pUC |
| 感受态 | Stbl3 |
| 培养温度 | 37度 |
| 质粒宿主 | 哺乳细胞 |
| 质粒用途 | 基因敲除 |
| 片段类型 | CRISPR,Cas9,sgRNA |
| 片段物种 | 小鼠 |
| 原核抗性 | Amp |
| pCMV-VSTM5(human)-3×FLAG-Neo |
| pCMV-BUB1(human)-Myc-Neo |
| pCMV-EGFP-ABCC2(human)-L1211P-Puro |
| pCMV-HDAC7(rat)-3×FLAG-Neo |
| pCMV-CCN4(human)-3×FLAG-Neo |
| pCMV-VASH2(human)-3×FLAG-Neo |
| pCMV-ANGPT1(human)-3×FLAG-Neo |
| pCMV-ZDHHC9(mouse)-EYFP-Neo |
| pCMV-BSG(human)-3×FLAG-Neo |
| pCMV-BPNT1(human)-Myc-FLAG-Neo |
| pCMV-mCherry-TRAM1(human)-3×HA-Neo |
| pCMV-PTPN21(human)-3×HA-Neo |
| pCMV-TMCC3(human)-3×FLAG-Neo |
| pU6-NONO(mouse)-sgRNA1-sgRNA2-sgRNA3-CBH-Cre-HA |
| pCMV-MIF(mouse)-3×FLAG-Puro |
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文献和实验RNAi Knockdown of Transcription Factor Pu.1 in the Differentiation of Mouse Embryonic Stem Cells
types. Mouse ES cells can be regarded as a versatile biological tool that has led to major advances in our understanding of cell and developmental biology. To study specific gene function in early developmental events, gene knockout approaches
Blastocyst embryo transfer into pseudopregnant recipient female mouse（图）
and exhibit bad planes of anesthesia when heavy. This is also a very inexpensive mouse to use. As an alternate, another strain I have used with considerable success is B6D2F1. These mice are hardy and display hybrid vigor. The Equipment For surgery
PROTOCOL General anesthesia inject i.p. 250µl/mouse of the ANESTHETIC mixture for verify the anesthetic effect check plantar reflexes (animal must be unresponsive to squeezing hind feet) Preparation
技术资料
