Sino Biological 重组新冠病毒(B.1.1.7+E484K)S1+S2 trimer蛋白(ECD,His标签) 高纯度高活性 新冠S蛋白

重组新冠病毒 (B.1.1.7+E484K) S1+S2 trimer 蛋白 (ECD, His标签)（产品说明）
蛋白名称：SARS-CoV-2 (B.1.1.7 + E484K) Spike S1+S2 trimer Protein (ECD, His tag)
蛋白构建：A DNA sequence encoding the SARS-CoV-2 Spike S1+S2 (YP_009724390.1, with mutations T19R, E156G, DEL157-158, L452R, T478K, D614G, P681R, D950N,F817P, A892P, A899P,A942P, K986P, V987P and furin cleavage site mutants) expressed with the bacteriophage T4 fibritin and a polyhistidine tag at the C-terminus. The mutations were identified in the SARS-CoV-2 variant (known as variant B.1.1.7 +E484K).
表达宿主：HEK293 Cells
蛋白纯度：> 90 % as determined by SDS-PAGE. > 95 % as determined by SEC-HPLC.
蛋白活性：Immobilized human ACE2 protein (mFc tag)(Cat:10108-H05H) at 2 μg/mL (100 μL/well) can bind SARS-CoV-2 (B.1.1.7 + E484K) Spike S1+S2 trimer Protein (ECD, His tag)(Cat:40589-V08H17), the EC50 is 5-30 ng/mL.
蛋白内毒素：< 1.0 EU per μg protein as determined by the LAL method.
预测N端：Val 16
蛋白分子量：The recombinant SARS-CoV-2 Spike S1+S2 consists of 1227 amino acids and predicts a molecular mass of 136.05 kDa.
蛋白NP号：YP_009724390.1
蛋白氨基酸序列：Met1-Gln1208
蛋白标签：C-His
蛋白保存条件：Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.