- ￥6470
- Sino Biological
- 北京
- 40830-V08H
- 2026年01月26日
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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
-20℃ to -80℃
- 保质期：
12个月
- 英文名：
Human respiratory syncytial virus (RSV) (strain A2) Glycoprotein G Protein (His Tag)
- 库存：
99
- 供应商：
北京义翘神州科技股份有限公司
- 规格：
100 µg
蛋白名称：Human respiratory syncytial virus (RSV) (strain A2) Glycoprotein G Protein (His Tag)
蛋白构建：A DNA sequence encoding the human respiratory syncytial virus (RSV) (strain A2) glycoprotein G (P03423) (Ser64-Gln298) was expressed with a polyhistidine tag at the C-terminus.
表达宿主：HEK293 Cells
蛋白纯度：> 95 % as determined by SDS-PAGE.
蛋白活性：0
蛋白内毒素：< 1.0 EU per μg protein as determined by the LAL method.
预测N端：Ser 64
蛋白分子量：The recombinant human respiratory syncytial virus (RSV) (subtype A2) Glycoprotein G consists of 246 amino acids and predicts a molecular mass of 26.97 kDa. It migrates as an approximately 107.50 and 55.70 kDa band in SDS-PAGE under reducing conditions.
蛋白NP号：P03423
蛋白氨基酸序列：Ser64-Gln298
蛋白标签：C-His
蛋白保存条件：Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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文献和实验2, Kimura, T; et al. Antigen-dependent interplay of formulation, systemic innate responses, and antibody responses to multi-component replicon RNA vaccination. Molecular therapy. Nucleic acids, PMID: 40606648
Site-Specific Chemical Modification of a Glycoprotein Fragment Expressed in Yeast
Site-specific modification of glycoproteins has wide application in both biochemical and biophysical studies. This method describes the conjugation of synthetic molecules to the N-terminus of a glycoprotein fragment, viz., human
特异性结合的单克隆抗体1。与Rho1D4抗体结合的表位可以作为针对膜蛋白的超高特异性纯化标记。通过基因修饰可在欲研究的目标（膜）蛋白C端加上Rho1D4标记。一旦加载上该序列，即可用装载有Rho1D4抗体的亲和基质去捕获目标蛋白，随后添加Rho1D4肽，通过竞争性结合基质抗体的方式来洗脱目标蛋白。采用这种方法，相比改变pH值等其他洗脱方式，更加地温和。图1：假设有3个跨膜域的膜蛋白。Rho1D4标签加到膜蛋白C端，它的序列是T-E-T-S-Q-V-A-P-A2） Rho1D4的历史Rho1D4的表位
(RSV) attachment glycoprotein (G), which involve the use of lectins and anti-carbohydrate antibodies. Given the role of the G glycoprotein in RSV antigenicity, we also describe procedures based on Western blotting to determine the effect of G protein
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