Treponema pallidum (E.coli, st

Recombinant Treponema pallidum (strain Nichols) TP0453

 

Catalog #: DRA179    Derived From: E.coli

 

Description: 

 

Recombinant Treponema pallidum (strain Nichols) Outer Membrane Protein TP0453 is produced by our E.coli expression system and the target gene encoding Cys25-Ser287 is expressed with a 6His tag at the N-terminus.

Accession: O67998

Known as: Outer membrane protein TP0453; TPANIC_0453; 30kLP; TP_0453; TP0453

 

Quality Control:

Mol Mass: 31.5kDa    AP Mol Mass: 30kDa, reducing conditions

Purity: Greater than 95% as determined by reducing SDS-PAGE.

 

Formulation:

Supplied as a 0.2 μm filtered solution of 20mM Tris-HCl, 8M Urea, 10mM β-Mercaptoethanol, pH8.0

 

Shipping:

The product is shipped on dry ice pack.

Upon receipt, store it immediately at the temperature listed below.

 

Storage:

Reconstituted protein solution should be stored at  ≤ -20°C.

 

Purification: 
Affinity purification chromatography

 

 

Application:
Immunogen, calibrator or standard.

 

Background:

TP0453 is the only lipoprotein in T. pallidum known to be associated with the outer membrane (OM). Syphilis is a multistage, sexually transmitted illness caused by the spirochetal pathogen Treponema pallidum, an extracellular, highly motile bacterium. Membrane integration of the TP0453 polypeptide was found to increase membrane permeability, suggesting the molecule functions in a porin-like manner.TP0453 is a novel bacterial lipoprotein whose amphiphilic behavior may contribute to T. pallidum OM biogenesis. And TP0453 is also a novel type of bacterial outer membrane protein which may render the T. pallidum outer membrane permeable to nutrients while remaining inaccessible to antibody.

 

 

FOR RESEARCH OR FURTHER MANUFACTURING USE ONLY