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- 博尔森/BIOESN
- BES21654KB
- 中国
- 2025年07月15日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
50
- 英文名：
β-Actin mouse monoclonal antibody
- 供应商：
上海博尔森生物科技有限公司
- 保存条件：
-20℃
- 规格：
100μL
β-Actin mouse monoclonal antibody
β-Actin is one of six different actin isoforms that have been identified. The actin molecules found in cells ofvarious species and tissues tend to be very similar in their immunological and physical properties. Therefore, Antibodies against β-Actin are useful as loading controls for Western Blotting. However it should be noted thatlevels of β-Actin may not be stable in certain cells. For example, expression of β-Actin in adipose tissue is verylow and therefore β-Actin should not be used as loading control for these tissues.
Product Name: β-Actin mouse monoclonal antibody
Clone Number: 6B8
Isotype: IgG1
Immunogen: Full-length β-Actin protein of human. Tested applications: WB IHC IF
Recommended dilutions: WB: 1:5000-2000 IHC: 1:200-100 IF: 1:200-100
Optimal dilutions/concentrations should be determined by the end user. Molecular Weight: 43KD
Form: Liquid
Concentration: 1mg/ml in PBS, pH 7.4, containing 0.02% sodium azide and 50% glycerol
Storage: β-Actin antibody is stable for 1 month at 2-8°C. For long term storage, Aliquot and store at -20°C or
below. Avoid repeated freezing and thawing cycles. Reactivity: Human, Monkey, Mouse, Rat, hamster, Dog, Spodoptera frugiperda, chicken, Zebrafish
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文献和实验Monoclonal Antibody Production
and screening of the hybridomas: 1. Bleeding Mice (1) Place the mouse in a mouse restrainer. (2) Sterilize the tail with 70% ethanol. (3) With a razor blade,nip off the last 2 mm of the tip of the tail. (4) Using a milking motion,pull blood down and let
指仅对一种抗原决定基的纯粹的抗体。这类抗体不可能通过将抗原 胞的 Hybridoma，在试管内制成了纯粹的单克隆抗体。用某种抗原使动物产生免疫感应，对应于抗原之不同部分可制成各种不同的抗体复合体。然而，因为一个产生抗体的细胞，只能产生一种抗体，所以骨髓肿瘤细胞与产生抗体细胞间的细胞杂种，可形成边产生抗体边增殖的 hybridoma，将每个杂种细胞进行克隆（无性繁殖系）培养，则一个克隆的细胞群只由单种类产生同一抗体的细胞所组成，这样得到的就是单克隆抗体。此抗体在测定
常用的β-actin 引物序列 human actin f ctc cat cct ggc ctc gct gt human actin r gct gtc acc ttc acc gtt cc product size:268 rabbit actin r agt gcg acg tgg aca tcc g rabbit actin f tgg ctc taa cag tcc gcc tag product size:295 mouse actin r cgt
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