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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃.
- 保质期:
Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.
- 英文名:
Recombinant Mouse Ras-related protein Rab-44 (Rab44), partial
- 库存:
200
- 供应商:
武汉华美生物工程有限公司
- 规格:
20μg
英文名称:
Recombinant Mouse Ras-related protein Rab-44 (Rab44), partial品名简称:
Recombinant Mouse Rab44 protein, partial货号:
CSB-EP806990MO规格:
20μg价格:
2328LC-MS/Western Blot:
/SDS-PAGE:
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.纯度:
Greater than 85% as determined by SDS-PAGE.内毒素:
Not test生物活性:
/基因名:
Rab44Uniprot No.:
Q8CB87别名:
/种属:
Mus musculus (Mouse)蛋白长度:
Partial来源:
E.coli分子量:
24.5 kDa表达区域:
785-937aa氨基酸序列:
LYHVVFLGDSNVGKTSFLHLLHHDAFATGLTATVGVDFRVKNLLVDNKTFALQLWDTAGQERYHSLTRQLLRKAEGVVLMYDVTSQESFTHVRYWLDCLQDAGVEGVAMVLLGNKMDCEEERQVPTEAGRRLAQELGVSFGECSAALGHNILE蛋白标签:
N-terminal 10xHis-tagged and C-terminal Myc-tagged产品提供形式:
Liquid or Lyophilized powder缓冲液:
If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.储存条件:
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.保质期:
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.货期:
3-7 business days注意事项:
Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.功能1:
/功能2:
/文献:
"Rab44, a novel large Rab GTPase, negatively regulates osteoclast differentiation by modulating intracellular calcium levels followed by NFATc1 activation." Yamaguchi Y., Sakai E., Okamoto K., Kajiya H., Okabe K., Naito M., Kadowaki T., Tsukuba T. Cell Mol Life Sci 75:33-48(2018)研究领域:
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文献和实验PNAS:RAS 抑制剂又添一员,新型蛋白模拟物或为癌症治疗
的细胞靶点Ras 蛋白由 150 多个成员组成,包括 Ras、Rho、Rab、Arf 和 Ran 亚家族。预计 Ras-Sos 复合物可能有多种组合模式,比如 Rab-Sos、Ran-Sos、Ras、Sos 等,目前尚无法知道 Sos 可能与哪些 Ras 家族成员结合。为了确定 CHDSos-5 的结合部位,研究团队对 H358 肺癌细胞进行荧光标记,然后通过基于蛋白质组学方法来富集相互作用因子及特征。研究团队共鉴定出 143 个蛋白质靶点:K-Ras、Ran 和几种 Ras 相关蛋白(RAB
上释放出来,取而代之的是GTP,从而将Ras激活,GEF的活性受生长因子及其受体的影响。另一个控制Ras蛋白活性的是GTP酶激活蛋白(GTPase activating protein, GAP),存在于正常细胞中,主要作用是激活Ras蛋白的GTP酶,将结合在Ras蛋白上的 GTP水解成GDP,成为失活型的 Ras蛋白—GDP。所以在正常情况下,Ras蛋白基本上都与 GDP结合在一起,定位在细胞质膜内表面上。
Nonradioactive Methods for Detecting Activation of Ras-Related Small G Proteins
active form of Ras -related G proteins involved immunoprecipitation of 32 P-labeled protein followed by separation of the labeled GTP/GDP bound to GTPase. A new method based on the large affinity difference of the GTP- and GDP-bound form of Ras proteins
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