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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃.
- 保质期:
Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.
- 英文名:
Recombinant Chlamydophila psittaci Small cysteine-rich outer membrane protein omcA (omcA)
- 库存:
200
- 供应商:
武汉华美生物工程有限公司
- 规格:
20μg
英文名称:
Recombinant Chlamydophila psittaci Small cysteine-rich outer membrane protein omcA (omcA)品名简称:
Recombinant Chlamydophila psittaci omcA protein货号:
CSB-EP315817CJB规格:
20μg价格:
2328LC-MS/Western Blot:
/SDS-PAGE:
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.纯度:
Greater than 90% as determined by SDS-PAGE.内毒素:
Not test生物活性:
/基因名:
omcAUniprot No.:
P0CZ19别名:
(Small-CRP)(9 kDa cysteine-rich lipoprotein)(9KD-CRP)种属:
Chlamydophila psittaci (Chlamydia psittaci)蛋白长度:
Full Length of Mature Protein来源:
E.coli分子量:
14.6 kDa表达区域:
20-87aa氨基酸序列:
CCRIVDCCFEDPCAPKPCNPCGNKKDKGCSPCGVYTPSCSKPCGSECNPGVQGPQAKGCTSLDGRCKQ蛋白标签:
N-terminal 10xHis-tagged and C-terminal Myc-tagged产品提供形式:
Liquid or Lyophilized powder缓冲液:
If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.储存条件:
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.保质期:
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.货期:
13-23 business days注意事项:
Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.功能1:
In elementary bodies (EBs, the infectious stage, which is able to survive outside the host cell) provides the structural integrity of the outer envelope through disulfide cross-links with the large cysteine-rich periplasmic protein and the major outer membrane porin. It has been described in publications as the Sarkosyl-insoluble COMC (Chlamydia outer membrane complex), and serves as the functional equivalent of peptidoglycan.功能2:
/文献:
"The CrP operon of Chlamydia psittaci and Chlamydia pneumoniae." Watson M.W., Clarke I.N., Everson J.S., Lambden P.R. Microbiology 141:2489-2497(1995)研究领域:
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文献和实验Labeling Gizzard Myosin with Tetramethylrhodamine Iodoacetamide (Primarily 17kDa Light Chain)
pellet in a small volume of buffer 1 to obtain 15-20 mg/ml concentration.Dialyze against 500 ml of buffer 1 for >=2 hours. 4.Measure volume.Dilute with dialysis buffer to obtain a myosin solution of 12-15 mg/ml. 5.Get an aliquot of IATR,dissolve in DMF
( 500 kDa) and remains with each peptide throughout the analysis. This can make database searching more difficult, especially for small peptides with limited sequence (4, 65). Sensitivity may also be of concern since tagged peptides derived from low
注意的是,两种配体的洗脱和再生buffer稍有差别,使用的时候还需注意! 系统特点 高达 pM 范围的亲和力(KD up to 6.2 x 10-11 M) 纯化树脂材质稳定 小标签 (8-28 aa)无需移除,不影响蛋白折叠和功能 极高的蛋白纯度(纯度>95%) 应用于多种目标蛋白检测系统:Western blot, ELISA, 免疫荧光, FACS等 兼容多种缓冲条件:高盐,洗涤剂,金属离子,螯合剂,还原剂 适用于多种蛋白的纯化 Twin-Strep-tag®标签蛋白固定在界面上时 (SPR
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