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文献和实验Immunofluorescent Staining of Mouse and Rat Leukocytes
-up, please see description in "Procedure for Setting Compensation for Multi-Color Flow Cytometric Analysis". For reducing FcgII/IIIR-mediated antibody binding (or binding of SAv-PE or SAv-Cy-Chrome) which could contribute to background, the use of anti-mouse CD32/CD
Cell Surface Immunofluorescence Staining Protocol
. In the mouse, purified anti-mouse CD16/CD32 antibody specific for Fcγ R III/II (BioLegend Cat. #101302, clone 93) can be used to block nonspecific staining of antibodies. In this case, block Fc receptors by pre-incubating cells with 5-10 μg/ml purified anti-CD
Expanding Mouse Ventricular Cardiomyocytes Through GSK‐3 Inhibition
: Donkey anti‐mouse, Alexa 488 (Invitrogen, cat. no. A‐21202, 1:400) Donkey anti‐rabbit, Alexa 594 (Invitrogen, cat. no. A‐21206, 1:400
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