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- 2025年09月25日
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文献和实验Phycoerythrin conjugation protocol
and Centricon concentrators. Add 0.5% sodium azide. Store at 4 °C protected from light. Do not freeze. Figure 1. Analysis of column eluate fractions from PE conjugation of monoclonal antibody IB4 (murine IgG2a, anti-human CD
Method for Purification of Human Hematopoietic Stem Cells by Flow Cytometry
Human hematopoietic stem cells (HSCs) and progenitors can be isolated by enriching for a rare cell population with a combination of monoclonal antibodies (MAbs). Such an isolation scheme involves multi-step procedures including
Immunofluorescence Staining of Human Cells by Lysed Whole Blood Method
to each tube. Centrifuge for 5 minutes at 1000 rpm (200 x g). Remove supernatant by aspiration and vortex. If third step is not required, proceed to step 18. For third step, add Sav-PE to each tube and vortex gently. Repeat steps 11-15
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