- ￥760 - 1910
- Proteintech
- CL488-65077
- 2025年10月13日
- IF/ICC, FC
- Rat
- Mouse
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
None
- 亚型：
IgG2b, kappa
- 保存条件：
Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.
- 克隆性：
Monoclonal
- 标记物：
CoraLite® Plus 488 Fluorescent Dye
- 适应物种：
Mouse
- 保质期：
详见产品包装
- 库存：
充足
- 宿主：
Rat
- 应用范围：
IF/ICC, FC
- 靶点：
CD3
- 抗体英文名：
CoraLite® Plus 488 Anti-Mouse CD3 (17A2)
- 抗体名：
CoraLite® Plus 488 Anti-Mouse CD3 (17A2)
- 规格：
25ug/100ug
| 规格： | 25ug | 产品价格： | ￥760.0 |
|---|---|---|---|
| 规格： | 100ug | 产品价格： | ￥1910.0 |
经过测试的应用
| Positive IF/ICC detected in | mouse splenocytes |
| Positive FC detected in | mouse splenocytes |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Immunofluorescence (IF)/ICC | IF/ICC : 1:250-1:1000 |
| Flow Cytometry (FC) | FC : 0.5 ug per 10^6 cells in 100 μl suspension |
| This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL488-65077 targets CD3 in IF/ICC, FC applications and shows reactivity with Mouse samples.
| 经测试应用 | IF/ICC, FC |
| 文献引用应用 | FC |
| 经测试反应性 | Mouse |
| 文献引用反应性 | mouse |
| 免疫原 |
fusion protein |
| 宿主/亚型 | Rat / IgG2b, kappa |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | CD3 antigen, epsilon polypeptide |
| 别名 | AI504783, CD3, Cd3e, CD3epsilon, T3e |
| GenBank蛋白编号 | BC098236 |
| 基因名称 | CD3ε |
| Gene ID (NCBI) | 12501 |
| RRID | AB_2883163 |
| 偶联类型 | CoraLite® Plus 488 Fluorescent Dye |
| 最大激发/发射波长 | 493 nm / 522 nm |
| 形式 | Liquid |
| 纯化方式 | Affinity purification |
| UNIPROT ID | P22646 |
| 储存缓冲液 | PBS with 0.09% sodium azide and 0.5% BSA, pH 7.3. |
| 储存条件 | Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment. |
背景介绍
CD3 is a multimeric protein associated with the T-cell receptor (TCR) to form a complex involved in antigen recognition and signal transduction (PMID: 15885124). CD3 is composed of CD3γ, δ, ε, and ζ chains (PMID: 1826255). It is expressed by thymocytes in a developmentally regulated manner, T cells, and some NK cells (PMID: 3289580). The TCR recognizes antigens bound to major histocompatibility complex (MHC) molecules. TCR-mediated peptide-MHC recognition is transmitted to the CD3 complex, leading to the intracellular signal transduction (PMID: 11985657).
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文献和实验| Species | Application | Title |
|---|---|---|
| mouse | FC | Int J Biol Macromol Polysaccharide-mediated combination therapy enhances anti-tumor effects by promoting the immune cycle of immunogenic cell deathAuthors - Xiang-Long Yu |
Intracellular Immunofluorescent Staining for Flow Cytometry
PBMCs were stimulated with TPA/ionomycin in the presence of monensin for 5 hours. The cells were fixed and permeabilized and intracellularly stained with anti-human CD4, clone RPA-T4, (Cat. No. 17-0049 ) and Alexa Fluor® 488 anti-human IL-17 (Cat. No. 53
：Catalog NumberProduct NameProduct TypeSizeApplication4408SAnti-mouse IgG (H+L), F(ab')2 Fragment (Alexa Fluor® 488 Conjugate)Secondary Antibody250µlIF-IC F HCA4409SAnti-mouse IgG (H+L), F(ab')2 Fragment (Alexa Fluor® 555 Conjugate)Secondary
基洗涤3遍，加入70%冷乙醇固定，4℃过夜。PBS洗涤3次，加入PI染液(生理盐水 129.6mL，PI 10mg，RNA酶2mg，TritonX-100 1mL，枸橼酸钠200mg，加双蒸水至200mL)，4℃避光染色30min，用FCM检测，记录激发波长488nm处的红色荧光。1.2.4 γδT细胞杀伤活性检测 取培养10d的γδT细胞和对数生长期SGC-7901、SW-1990、SW-480、SW-1116和LOVO细胞分别配成2×108/L，加入6孔细胞培养板中，每孔3mL，培养24h后加
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