- ￥1350 - 3000
- Proteintech
- 28459-1-AP
- 2025年10月13日
- IHC, IF/ICC, IF-P, ELISA
- Rabbit
- human
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
Recombinant human COL2A1 protein
- 亚型：
IgG
- 保存条件：
Store at -20°C. Stable for one year after shipment.
- 克隆性：
Polyclonal
- 标记物：
Unconjugated
- 适应物种：
human
- 保质期：
详见产品包装
- 库存：
充足
- 宿主：
Rabbit
- 应用范围：
IHC, IF/ICC, IF-P, ELISA
- 靶点：
Collagen Type II
- 抗体英文名：
Collagen Type II Polyclonal antibody
- 抗体名：
Collagen Type II Polyclonal antibody
- 规格：
50ul/100ul/150ul
| 规格： | 50ul | 产品价格： | ￥1350.0 |
|---|---|---|---|
| 规格： | 100ul | 产品价格： | ￥2200.0 |
| 规格： | 150ul | 产品价格： | ￥3000.0 |
经过测试的应用
| Positive IHC detected in | human lung tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | human lung cancer tissue |
| Positive IF/ICC detected in | ATDC-5 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Immunohistochemistry (IHC) | IHC : 1:800-1:3200 |
| Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
28459-1-AP targets Collagen Type II in WB, IHC, IF/ICC, IF-P, CoIP, ELISA applications and shows reactivity with human samples.
| 经测试应用 | IHC, IF/ICC, IF-P, ELISA |
| 文献引用应用 | WB, IHC, IF, CoIP |
| 经测试反应性 | human |
| 文献引用反应性 | human, mouse, rat, pig, rabbit, oryctolagus cuniculus (rabbit) |
| 免疫原 |
CatNo: Ag29265 Product name: Recombinant human COL2A1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-63 aa of BC007252 Sequence: MSAFAGLGPREKGPDPLQYMRADQAAGGLRQHDAEVDATLKSLNNQIESIRSPEGSRKNPART |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | collagen, type II, alpha 1 |
| 别名 | COL2, COL2A1, Alpha 1 type II collagen, Alpha-1 type II collagen, ANFH |
| 计算分子量 | 134 kDa |
| GenBank蛋白编号 | BC007252 |
| 基因名称 | COL2A1 |
| Gene ID (NCBI) | 1280 |
| RRID | AB_2881147 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P02458 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
The COL2A1 gene encodes the alpha-1 chain of type II collagen, a fibrillar collagen found primarily in cartilage and the vitreous humor of the eye. Type II collagen is essential for the formation of the extracellular matrix in cartilage and other connective tissues, providing structure and strength to these tissues .
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文献和实验| Species | Application | Title |
|---|---|---|
| mouse,human | IHC,IF | Nature A plant-derived natural photosynthetic system for improving cell anabolismAuthors - Pengfei Chen |
| rat | IF | Adv Mater Injectable Microgels with Hybrid Exosomes of Chondrocyte-Targeted FGF18 Gene-Editing and Self-Renewable Lubrication for Osteoarthritis TherapyAuthors - Manyu Chen |
| mouse,rat | IHC,IF | Bioact Mater Synergizing adaptive immunity and regenerative signals to enhance osteochondral defects repairAuthors - Changjian Lin |
| rat | IHC | Bioact Mater Engineering a stem cell-embedded bilayer hydrogel with biomimetic collagen mineralization for tendon-bone interface healingAuthors - Tingyun Lei |
| rat | IF | Nat Commun Injectable ECM-mimetic dynamic hydrogels abolish ferroptosis-induced post-discectomy herniation through delivering nucleus pulposus progenitor cell-derived exosomesAuthors - Wenkai Wang |
| human,rat | WB | Adv Sci (Weinh) Mechanical Load-Induced Upregulation of Talin2 through Non-Canonical Deubiquitination of OTUB1 Drives Facet Joint Osteoarthritis PathogenesisAuthors - Yizhen Huang |
Immunoassays for Collagenase-Mediated Cleavage of Type I and II Collagens
This chapter describes the production and characterization of antibodies raised against neoepitopes in collagenase-cleaved collagen. It also details the development, validation, and use of immunoassays using such antibodies to measure
for assaying collagen degradation in situ will be described. Although the most abundant collagen in the body is type I collagen (found, for example, in skin, bone, ligaments and tendons), much of this chapter will focus on type II collagen, the major collagen
Collagen-Induced Arthritis in Mice
with autologous or heterologous type II collagen in complete Freund’s adjuvant and susceptibility to the disease is strongly associated with major histocompatibility complex class II genes. The development of collagen-induced arthritis is associated with strong T
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