- ￥1350 - 3000
- Proteintech
- 10912-1-AP
- 2025年10月13日
- WB, IHC, ELISA
- Rabbit
- human, mouse, rat
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
Recombinant human NUDT11 protein
- 亚型：
IgG
- 保存条件：
Store at -20°C. Stable for one year after shipment.
- 克隆性：
Polyclonal
- 标记物：
Unconjugated
- 适应物种：
human, mouse, rat
- 保质期：
详见产品包装
- 库存：
充足
- 宿主：
Rabbit
- 应用范围：
WB, IHC, ELISA
- 靶点：
NUDT3/4/10/11
- 抗体英文名：
NUDT3/4/10/11 Polyclonal antibody
- 抗体名：
NUDT3/4/10/11 Polyclonal antibody
- 规格：
50ul/100ul/150ul
| 规格： | 50ul | 产品价格： | ￥1350.0 |
|---|---|---|---|
| 规格： | 100ul | 产品价格： | ￥2200.0 |
| 规格： | 150ul | 产品价格： | ￥3000.0 |
经过测试的应用
| Positive WB detected in | mouse brain tissue, human brain tissue, rat brain tissue |
| Positive IHC detected in | mouse testis tissue, human ovary tumor tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| Immunohistochemistry (IHC) | IHC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
10912-1-AP targets NUDT3/4/10/11 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, ELISA |
| 文献引用应用 | WB, IHC |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | mouse |
| 免疫原 |
CatNo: Ag1346 Product name: Recombinant human NUDT11 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-164 aa of BC009942 Sequence: MKCKPNQTRTYDPEGFKKRAACLCFRSEREDEVLLVSSSRYPDRWIVPGGGMEPEEEPGGAAVREVYEEAGVKGKLGRLLGVFEQNQDRKHRTYVYVLTVTELLEDWEDSVSIGRKREWFKVEDAIKVLQCHKPVHAEYLEKLKLGGSPTNGNSMAPSSPDSDP |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | nudix (nucleoside diphosphate linked moiety X)-type motif 11 |
| 别名 | APS1, ASP1, DIPP 3 beta, DIPP3 beta, DIPP3b, DIPP3beta, hAps1, hDIPP3beta, Nudix motif 11, NUDT11 |
| 计算分子量 | 19 kDa |
| 观测分子量 | 19 kDa |
| GenBank蛋白编号 | BC009942 |
| 基因名称 | NUDT11 |
| Gene ID (NCBI) | 55190 |
| RRID | AB_2153836 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q96G61 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
NUDT11 (nucleoside diphosphate linked moiety X-type motif 11), also named as DIPP3B or APS1, belongs to the subgroup of phosphohydrolases that preferentially attack diphosphoinositol polyphosphates (PMID: 12105228, 12689335). Five human isoforms of DIPP have been found and named as DIPP type 1(NUDT3), type 2α/β(NUDT4), type 3α(NUDT10) and type 3β(NUDT11), which have the similar molecular weight and high homology (PMID: 12105228). This antibody can recognize all the 5 isoforms of DIPP (NUDT3/4/10/11).
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文献和实验| Species | Application | Title |
|---|---|---|
| mouse | WB,IHC | Sci Rep Construction of a diagnostic model utilizing m7G regulatory factors for the characterization of diabetic nephropathy and the immune microenvironmentAuthors - Jingying Zhong |
Expression Library Screening (Procaryotic) Using AP-Fusion Proteins
vector and can not be used for in vivo excision. Primers for gt11 are available from Sigma (forward primer: #P1680, reverse primer: #P1805). Growing up host bacteria Reagents: 1M MgSO4 (100 x
Using ProHits to Store, Annotate, and Analyze Affinity Purification–Mass Spectrometry (AP‐MS) Data
Figure 8.16.11 Uploading search results. (A ) Once a Bait , Experiment , and Sample have been created, the Upload Search Results function can be used to import search results from the Mascot, X!Tandem
的Caspase-3及对底物多聚（ADP-核糖）聚合酶[poly（ADP-ribose）polymerase，PARP]等的裂解。方法： 收集细胞→PBS洗涤→抽提细胞裂解液→蛋白定量→SDS-PAGE电泳→硝酸纤维素膜或PVDF膜转移→5%脱脂奶粉封闭，室温1.5~2h或4°C过夜→Caspase-3多抗或单抗室温反应1~2h或4°C过夜→TBS-T（含0.05% Tween 20的TBS）洗3次，5~10min/次→HRP-标记的羊抗鼠IgG或AP标记的羊抗鼠IgG室温反应1~2h→ TBS-T洗3次
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