CF647 酪酰胺 96022

CF® Dye tyramide conjugates are used for tyramide signal amplification (TSA), a method for high-density labeling of a target protein or nucleic acid in situ.

• High-density labeling of a target protein or nucleic acid for enhanced immunofluorescence sensitivity
• Especially suited for the detection of low abundance targets
• Detection sensitivity of over 100-fold compared to conventional procedures
• Enables multiplex multicolor detection, not limited by antibodies from the same host species
• Wide selection of bright, photostable and water-soluble CF® Dyes, excellent options for fluorescent labeling
• CF®754 Tyramide is a unique near-IR conjugate compatible with automated staining

We also offer Ready-to-Use Tyramide Amplification Buffer, Tyramide Amplification Buffer Plus (an improved formulation for enhanced TSA sensitivity), and CF® Dye Tyramide Amplification Kits.

Superior CF® Dyes

Biotium’s next-generation CF® Dyes were designed to be highly water-soluble with advantages in brightness and photostability compared to Alexa Fluor®, DyLight®, and other fluorescent dyes. Our CF® Dye Tyramide conjugates are available in 21 colors. Learn more about CF® Dyes.

CF®754 Tyramide: A Stable Near-IR Tyramide for Automated Staining

The stability of CF®754 Tyramide in the amplification buffer makes it suitable for staining in automated systems (ie. BOND RX) where the dye may be added to the buffer in advance for several hours to overnight without degradation. CF®754 Tyramide was developed as a new and improved version of CF®750 Tyramide which is not stable in the oxidizing amplification buffer, particularly if stored premixed with buffer for several hours. However, CF®750 Tyramide may still be used if added to the amplification buffer just before use.

Tyramide Signal Amplification

TSA is a highly sensitive method for differential gene or protein analysis or detection of low-abundance targets, in fluorescent ICC, IHC, and FISH applications. An antibody- or streptavidin-HRP conjugate catalyzes the deposition of fluorescent dye/biotin tyramides on tyrosine residues on and adjacent to a target protein or nucleic acid sequence in situ. This results in high-density labeling of the target and significantly improves the detection sensitivity up to 100-fold compared to conventional methods. TSA is particularly advantageous for fluorescence detection in human tissue, where conventional ICC or FISH often fails to provide adequate signal over autofluorescence background. In applications where increased sensitivity is not required, TSA enables the use of significantly lower antibody or probe concentrations for the same level of detection sensitivity thereby reducing issues of non-specific binding or cross-reactivity. Furthermore, since binding of the tyramide label is covalent, a large number of targets can be detected in the same sample using multiple rounds of sequential TSA, in which the availability of antibodies from different host species is not a limitation. TSA also can be easily integrated with conventional immunostaining. Learn more about Tyramide Signal Amplification.

CF® Dye Tyramides

Product	Ex/Em	Size	Catalog No.
CF®350 Tyramide	347/448 nm	0.5 mg	92170
CF®405S Tyramide	404/431 nm	0.5 mg	92197
CF®405M Tyramide	408/452 nm	0.5 mg	96057
CF®405L Tyramide	395/545 nm	0.5 mg	92198
CF®430 Tyramide	426/498 nm	0.5 mg	96053
CF®488A Tyramide	490/515 nm	0.5 mg	92171
CF®514 Tyramide	516/548 nm	0.5 mg	92199
CF®532 Tyramide	527/558 nm	0.5 mg	96066
CF®543 Tyramide	541/560 nm	0.5 mg	92172
CF®550R Tyramide	551/577 nm	0.5 mg	96077
CF®555 Tyramide	555/565 nm	0.5 mg	96021
CF®568 Tyramide	562/583 nm	0.5 mg	92173
CF®583R Tyramide	586/609 nm	0.5 mg	96085
CF®594 Tyramide	593/614 nm	0.5 mg	92174
CF®620R Tyramide	617/639 nm	0.5 mg	92194
CF®640R Tyramide	642/662 nm	0.5 mg	92175
CF®647 Tyramide	650/665 nm	0.5 mg	96022
CF®660R Tyramide	663/682 nm	0.5 mg	92195
CF®680R Tyramide	680/701 nm	0.5 mg	92196
CF®750 Tyramide*	755/779 nm	0.5 mg	96052
CF®754 Tyramide	748/793 nm	0.5 mg	96090

* CF®750 Tyramide is not stable in TSA buffer, and should be added to the buffer immediately before performing the staining reaction.