- 询价
- Cell Signaling Technology
- 3090
- USA
- 2025年12月24日
- W, IP
- Rabbit
- H,M
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- 详细信息
- 文献和实验
- 技术资料
- 抗体英文名：
Pyk2 (H364) Antibody
- 抗原：
synthetic peptide corresponding to residues surrounding His364 of human Pyk2
- 应用范围：
W, IP
- 宿主：
Rabbit
- 供应商：
CST
- 适应物种：
H,M
- 级别：
详见MSDS文件
- 保质期：
详见说明书
- 库存：
大量
- 是否单克隆：
2
- 保存条件：
-20°c
- 规格：
100 ul (10 western blots)/carrier free & custom formulation / quantity
| 规格： | 产品价格： | ￥请询价 | |
|---|---|---|---|
| 规格： | 100 ul (10 western blots) | 产品价格： | ￥请询价 |
| 规格： | carrier free & custom formulation / quantity | 产品价格： | ￥请询价 |
pathway more info application references datasheet PDF MSDS PDF protocols
Applications Key: W=Western Blotting IP=Immunoprecipitation
Reactivity Key: H=Human M=Mouse
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.
| Applications | Reactivity | Sensitivity | MW (kDa) | Source |
|---|---|---|---|---|
| W IP | H M | Endogenous | 116 | Rabbit |
| Protocols |
|
|---|---|
| Specificity / Sensitivity | Pyk2 (H364) Antibody detects endogenous levels of total Pyk2 protein. This antibody does not cross-react with other related proteins. |
| Source / Purification | Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding His364 of human Pyk2. Antibodies are purified by protein A and peptide affinity chromatography. Western Blotting
Western blot analysis of extracts from Jurkat and Ramos cells using Pyk2 (H364) Antibody. |
| Background | Protein tyrosine kinase Pyk2, also called CAKβ, RAFTK and CADTK, is a nonreceptor tyrosine kinase structurally related to focal adhesion kinase (FAK) (1-4). Pyk2 is predominantly expressed in cells derived from hematopoietic lineages and in the central nervous system. Pyk2 is one of the signaling mediators for the G-protein-coupled receptors and MAP kinase signaling pathway. It plays an important role in cell spreading and migration (5-7).
|
| Application References | Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know ! |
| Companion Products |
For Research Use Only. Not For Use In Diagnostic Procedures. |
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文献和实验大小一致，具有特定的荧光强度，包被有适用于特定分析（如其它抗体或者可溶性蛋白）的特异性捕获抗体（Capture Antibody），提供了类似ELISA孔板的捕获表面。当微球和待测样品溶液混合后，微球上的特异性抗体就与样品（血清、血浆或者细胞培养液）中相应的抗原或蛋白结合，然后加入荧光标记的检测抗体，就会形成“三明治”夹心复合物。最后通过流式细胞仪对特异性目的蛋白进行检测。CBA的每种微球携带有不同强度的红色荧光，在流式细胞仪的FL3通道通过检测微球荧光强度的差异对目的蛋白定性；检测抗体带有PE
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