- 询价
- Cell Signaling Technology
- 8242
- USA
- 2025年07月14日
- W, IP, IHC-P, IF-IC, F, ChIP
- H,M,R,Hm,Mk,Dg
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- 详细信息
- 询价记录
- 文献和实验
- 技术资料
- 抗体英文名：
NF-κB p65 (D14E12) XP®️ Rabbit mAb
- 抗原：
synthetic peptide corresponding to residues surrounding Glu498 of human NF-κB p65/RelA protein
- 应用范围：
W, IP, IHC-P, IF-IC, F, ChIP
- 库存：
大量
- 供应商：
CST
- 级别：
详见MSDS文件
- 保质期：
详见说明书
- 适应物种：
H,M,R,Hm,Mk,Dg
- 是否单克隆：
单克隆
- 保存条件：
-20°c
pathway more info application references datasheet PDF MSDS PDF protocols
Applications Key: W=Western Blotting IP=Immunoprecipitation IHC-P=Immunohistochemistry (Paraffin) IF-IC=Immunofluorescence (Immunocytochemistry) F=Flow Cytometry ChIP=Chromatin IP
Reactivity Key: H=Human M=Mouse R=Rat Hm=Hamster Mk=Monkey Dg=Dog
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.
| Applications | Reactivity | Sensitivity | MW (kDa) | Isotype |
|---|---|---|---|---|
| W IP IHC-P IF-IC F ChIP | H M R Hm Mk Dg | Endogenous | 65 | Rabbit IgG |
| Protocols |
|
|---|---|
| Specificity / Sensitivity | NF-κB p65 (D14E12) XP® Rabbit mAb recognizes endogenous levels of total NF-κB p65/RelA protein. It does not cross react with other NF-κB/Rel family members. |
| Source / Purification | Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Glu498 of human NF-κB p65/RelA protein. Western Blotting
Western blot analysis of extracts from various cell lines using NF-κB p65 (D14E12) XP® Rabbit mAb. IHC-P (paraffin)
Immunohistochemical analysis of paraffin-embedded human chronic cholecystitis using NF-κB p65 (D14E12) XP® Rabbit mAb. IHC-P (paraffin)
Immunohistochemical analysis of paraffin-embedded HeLa cell pellets, control (left) or treated with hTNF-α #8902 (right), using NF-κB p65 (D14E12) XP® Rabbit mAb. Flow Cytometry
Flow cytometric analysis of HeLa cells using NF-κB p65 (D14E12) XP® Rabbit mAb (blue) compared to concentration matched Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (red). IF-IC
Confocal immunofluorescent analysis of HT-1080 cells, untreated (left) or treated with hTNF-α #8902 (20 ng/ml, 20 min) (right), using NF-κB p65 (D14E12) XP® Rabbit mAb (green). Actin filaments were labeled with DY-554 phalloidin (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye). Chromatin IP
Chromatin immunoprecipitations were performed with cross-linked chromatin from 4 x 106 HeLa cells treated with hTNF-α #8902 (30 ng/ml, 1 hr) and either 5 μl of NF-κB p65 (D14E12) XP® Rabbit mAb or 2 μl of Normal Rabbit IgG #2729 using SimpleChIP® Enzymatic Chromatin IP Kit (Magnetic Beads) #9003. The enriched DNA was quantified by Real-Time PCR using SimpleChIP® Human IκBα Promoter Primers #5552, human IL-8 promoter primers, and SimpleChIP® Human α Satellite Repeat Primers #4486. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin, which is equivalent to one. |
| Background | Transcription factors of the nuclear factor κ B (NF-κB)/Rel family play a pivotal role in inflammatory and immune responses (1,2). There are five family members in mammals: RelA, c-Rel, RelB, NF-κB1 (p105/p50), and NF-κB2 (p100/p52). Both p105 and p100 are proteolytically processed by the proteasome to produce p50 and p52, respectively. Rel proteins bind p50 and p52 to form dimeric complexes that bind DNA and regulate transcription. In unstimulated cells, NF-κB is sequestered in the cytoplasm by IκB inhibitory proteins (3-5). NF-κB-activating agents can induce the phosphorylation of IκB proteins, targeting them for rapid degradation through the ubiquitin-proteasome pathway and releasing NF-κB to enter the nucleus where it regulates gene expression (6-8). NIK and IKKα (IKK1) regulate the phosphorylation and processing of NF-κB2 (p100) to produce p52, which is then translocated to the nucleus (9-11).
|
| Application References | Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know ! |
| Companion Products |
For Research Use Only. Not For Use In Diagnostic Procedures. |
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文献和实验h）即可。2. 修复大法——不仅仅是「煮一煮」微波炉修复：简单易行效果好，CST 推荐使用微波炉完成修复。合适的修复液：根据抗体说明书使用合适的修复液。用柠檬酸修复后，切片需浸泡在修复液中，自然冷却；而用 EDTA 修复后，切片可直接从修复缸中取出，直接进行下一步。注：使用不同的修复方式和不同生产商的抗体检测人肺癌组织中 EGFR 的表达。第一排为 CST 的 EGF Receptor (D38B1) XP® Rabbit mAb（#4267），EDTA 的修复方式明显优于柠檬酸盐及胃蛋白
that can interact with the adaptor protein MyD88. p50 and p65 are the two most common DNA-binding subunits of the nuclear factor κ B (NFκ B) dimer, and have the ability to trigger the transcription of target genes that encode cytokine s, chemokines, proteins
的染色强度。获得合适的染色强度后，将载玻片置于蒸馏水 (dH2O) 中以阻止进一步显色。抗体：Phospho–Stat3(Tyr705)(D3A7) XP® Rabbit mAb #9145样本：石蜡包埋的人类乳腺肿瘤显色检测：利用 SignalStain® DAB Substrate Kit #8059（左），竞争者提供的 DAB（中和右）CST 的 IHC/IF 明星产品推荐：生化试剂：Catalog NumberProduct NameProduct
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