- 询价
- Cell Signaling Technology
- 2388
- USA
- 2025年07月12日
- W
- Rabbit
- H,M,R
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- 详细信息
- 文献和实验
- 技术资料
- 抗体英文名：
Phospho-IRS-1 (Ser636/639) Antibody
- 抗原：
synthetic phosphopeptide corresponding to residues surrounding serine 636/639 of human IRS-1
- 应用范围：
W
- 宿主：
Rabbit
- 级别：
详见MSDS文件
- 适应物种：
H,M,R
- 供应商：
CST
- 库存：
大量
- 保质期：
详见说明书
- 是否单克隆：
2
- 保存条件：
-20°c
- 规格：
100 ul (10 western blots)/carrier free & custom formulation / quantity
| 规格： | 产品价格： | ￥请询价 | |
|---|---|---|---|
| 规格： | 100 ul (10 western blots) | 产品价格： | ￥请询价 |
| 规格： | carrier free & custom formulation / quantity | 产品价格： | ￥请询价 |
pathway more info application references datasheet PDF MSDS PDF protocols
Applications Key: W=Western Blotting
Reactivity Key: H=Human M=Mouse R=Rat
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.
| Applications | Reactivity | Sensitivity | MW (kDa) | Source |
|---|---|---|---|---|
| W | H M R | Endogenous | 180 | Rabbit |
| Protocols |
|
|---|---|
| Specificity / Sensitivity | Phospho-IRS-1 (Ser636/639) Antibody detects endogenous levels of IRS-1 only when phosphorylated at Ser636/639. This antibody does not cross-react with other related phospho-proteins. |
| Source / Purification | Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding serine 636/639 of human IRS-1. Antibodies are purified by protein A and peptide affinity chromatography. Western Blotting
Western blot analysis of immunoprecipitates from 3T3-L1 adipocytes untreated and treated with insulin minus or plus and calf intestinal phosphatase (CIP) (upper) and of cell lysates from 3T3-L1 adipocytes stimulated with 100 nM insulin for various times (lower), using Phospho-IRS-1 (Ser636/639) Antibody. |
| Background | Insulin receptor substrate 1 (IRS-1) is one of the major substrates of the insulin receptor kinase (1). IRS-1 contains multiple tyrosine phosphorylation motifs that serve as docking sites for SH2-domain containing proteins that mediate the metabolic and growth-promoting functions of insulin (2-4). IRS-1 also contains over 30 potential serine/threonine phosphorylation sites. Ser307 of IRS-1 is phosphorylated by JNK (5) and IKK (6) while Ser789 is phosphorylated by SIK-2, a member of the AMPK family (7). The PKC and mTOR pathways mediate phosphorylation of IRS-1 at Ser612 and Ser636/639, respectively (8,9). Phosphorylation of IRS-1 at Ser1101 is mediated by PKCθ and results in an inhibition of insulin signaling in the cell, suggesting a potential mechanism for insulin resistance in some models of obesity (10).
|
| Application References |
Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know ! |
| Companion Products |
For Research Use Only. Not For Use In Diagnostic Procedures. |
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文献和实验Optimized Protocol to Make Phospho-Specific Antibodies that Work
, not simply its level of expression. In this review, we will discuss both the design of the phosphopeptide immunogen and immunization. The affinity purification of the phospho-specific antibody as well as the methods most suitable for characterizing
Using Phospho‐Motif Antibodies to Determine Kinase Substrates
comprising both the phosphorylated residue and the surrounding residues that determine kinase specificity, with degenerate residues taking up the remaining positions. Currently, several categories of phospho?motif antibody are commercially available
Absorption Control in Immunohistochemistry Using Phospho-Peptides Immobilized on Magnetic Beads
neutralization of phospho-specific antibodies with phospho-peptides immobilized on magnetic beads. This technique allows for sequestration of antibody–peptide complex from the incubation solution, minimizing the risk of formation of unblocked antibodies capable
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