P-Cadherin Antibody

2130:

Flow , Immunofluorescence , Immunoprecipitation , Western Blotting

P-Cadherin Antibody recognizes endogenous levels of total P-cadherin protein. The antibody does not cross-react with other cadherin family members.

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to human P-cadherin. Antibodies are purified using protein A and peptide affinity chromatography.

Western Blotting

Western blot anlaysis of extracts from A431, Panc1 and HUVE cells, using P-Cadherin Antibody.

IP

Immunoprecipitation of P-cadherin from A431 cell extracts, using P-Cadherin Antibody. Immunoblot was performed with the same antibody.

Flow Cytometry

Flow cytometric analysis of A431 cells, using P-Cadherin antibody (blue) compared to a nonspecific negative control antibody (red).

IF-IC

Immunofluorescent analysis of A431 cells, using P-Cadherin Antibody (left). Nuclei in the same cells were stained with DAPI (right).

IF-IC

Confocal immunofluorescent images of A431 cells labeled with P-Cadherin Antibody (green, left) compared to an isotype control (right). Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red). Blue pseudocolor = DRAQ™ (fluorescent DNA dye).

Cadherins are a superfamily of transmembrane glycoproteins that contain cadherin repeats of approximately 100 residues in their extracellular domain. Cadherins mediate calcium-dependent cell-cell adhesion and play critical roles in normal tissue development (1). The classic cadherin subfamily includes N-, P-, R-, B- and E-cadherins, as well as about ten other members that are found in adherens junctions, a cellular structure near the apical surface of polarized epithelial cells. The cytoplasmic domain of classical cadherins interacts with β-catenin, γ-catenin (also called plakoglobin), and p120 catenin. β-catenin and γ-catenin associate with α-catenin, which links the cadherin-catenin complex to the actin cytoskeleton (1,2). While β- and γ-catenin play structural roles in the junctional complex, p120 regulates cadherin adhesive activity and trafficking (1-4). E-cadherin is considered an active suppressor of invasion and growth of many epithelial cancers (1-3). Recent studies indicate that cancer cells have up-regulated N-cadherin in addition to loss of E-cadherin. This change in cadherin expression is called the "cadherin switch". N-cadherin cooperates with the FGF receptor, leading to overexpression of MMP-9 and cellular invasion (3). In endothelial cells, VE-cadherin signaling, expression, and localization correlate with vascular permeability and tumor angiogenesis (5,6). Expression of P-cadherin, which is normally present in epithelial cells, is also altered in ovarian and other human cancers (7,8).

1. Wheelock, M.J. and Johnson, K.R. (2003) Annu. Rev. Cell. Dev. Biol. 19, 207-235.
2. Christofori, G. (2003) EMBO J. 22, 2318-2323.
3. Hazan, R.B. et al. (2004) Ann. NY Acad. Sci. 1014, 155-163.
4. Bryant, D.M. and Stow, J.L. (2004) Trends Cell Biol. 14, 427-434.
5. Rabascio, C. et al. (2004) Cancer Res. 64, 4373-4377.
6. Yamaoka-Tojo, M. et al. (2006) Arterioscler. Thromb. Vasc. Biol. 26, 1991-1997.
7. Patel, I.S. et al. (2003) Int. J. Cancer 106, 172-177.
8. Sanders, D.S. et al. (2000) J. Pathol. 190, 526-530.

Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know !

• 4068 Pan-Cadherin Antibody
• 3195 E-Cadherin (24E10) Rabbit mAb
• 4061 N-Cadherin Antibody
• 7071 Phototope^® -HRP Western Blot Detection System, Anti-rabbit IgG, HRP-linked Antibody
• 7074 Anti-rabbit IgG, HRP-linked Antibody
• 7720 Prestained Protein Marker, Broad Range (Premixed Format)
• 7727 Biotinylated Protein Ladder Detection Pack
• 7003 20X LumiGLO^® Reagent and 20X Peroxide

For Research Use Only. Not For Use In Diagnostic Procedures.