- 询价
- Cell Signaling Technology
- 2272
- USA
- 2025年08月25日
- W, IF-IC, F
- Rabbit
- All
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- 详细信息
- 文献和实验
- 技术资料
- 抗体英文名：
Myc-Tag Antibody
- 抗原：
synthetic peptide corresponding to residues 410-419 of human c-Myc (EQKLISEEDL)
- 应用范围：
W, IF-IC, F
- 宿主：
Rabbit
- 库存：
大量
- 适应物种：
All
- 保质期：
详见说明书
- 级别：
详见MSDS文件
- 供应商：
CST
- 是否单克隆：
2
- 保存条件：
-20°c
- 规格：
100 ul (10 western blots)/carrier free & custom formulation / quantity
| 规格： | 产品价格： | ￥请询价 | |
|---|---|---|---|
| 规格： | 100 ul (10 western blots) | 产品价格： | ￥请询价 |
| 规格： | carrier free & custom formulation / quantity | 产品价格： | ￥请询价 |
pathway more info application references datasheet PDF MSDS PDF protocols
Applications Key: W=Western Blotting IF-IC=Immunofluorescence (Immunocytochemistry) F=Flow Cytometry
Reactivity Key: All=All species expected
Species cross-reactivity is determined by western blot.
| Applications | Reactivity | Sensitivity | Source |
|---|---|---|---|
| W IF-IC F | All | Transfected Only | Rabbit |
| Protocols |
|
|---|---|
| Specificity / Sensitivity | Myc-Tag Antibody detects recombinant proteins containing the Myc epitope tag. The antibody recognizes the Myc-tag fused to either the amino or carboxy terminus of targeted proteins in transfected cells. |
| Source / Purification | Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues 410-419 of human c-Myc (EQKLISEEDL). Antibodies are purified by protein A and peptide affinity chromatography. Western Blotting
Western blot analysis of cell extracts alone (lane 1), cells expressing Myc tagged to the carboxy terminus of the protein (lane 2) and Myc tagged to the amino terminus of the protein (lane 3), using Myc-Tag Antibody. Flow Cytometry
Flow cytometric analysis of COS cells, untreated (blue) or Myc transfected (green), using Myc-Tag Antibody. IF-IC
Confocal immunofluorescent analysis of COS cells transfected with a Myc-tagged protein (left) or untransfected (right) using Myc-Tag Antibody. Actin filaments have been labeled with DY-554 phalloidin (red). |
| Background | Epitope tags are useful for the labeling and detection of proteins using immunoblotting, immunoprecipitation, and immunostaining techniques. Because of their small size, they are unlikely to affect the tagged protein's biochemical properties. The Myc epitope tag is widely used to detect expression of recombinant proteins in bacteria, yeast, insect and mammalian cell systems (1).
|
| Application References |
Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know ! |
| Companion Products |
For Research Use Only. Not For Use In Diagnostic Procedures. |
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文献和实验Generation of Antibody Molecules Through Antibody Engineering
been overcome to a large extent using genetic-engineering techniques to produce chimeric mouse/human and completely human antibodies. Such an approach is particularly suitable because of the domain structure of the antibody molecule ( 2 ), where functional
The importance of antibody molecules was first recognized in the 1890s, when it was shown that immunity to tetanus and diphtheria was caused by antibodies against the bacterial exotoxins (1 ). Around the same time, it was shown that antisera
General comments: Antibodies, like most proteins, do not like to be freeze-thawed. Avoid repetitive freezing of your solution. The best way to store your antibody is to keep a high protein concentration (>1 mg/ml), add some protease
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